Nanowire devices for exosomal MicroRNA extraction

Satoru Ito, Takao Yasui, He Yong, Takeshi Yanagida, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Hiroshi Yukawa, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We fabricated nanowire devices, which had zinc oxide (ZnO) nanowires embedded in microchannels on PMMA substrate, to isolate exosomes from cell culture supernatant and extract MicroRNA (miRNA) from the isolated exosomes. The exosomes could be trapped by nanowires without any capturing molecules, such as antibodies against the exosomes. The exosomal miRNA could be extracted efficiently with little loss of the miRNA amount, which is comparable to that using a conventional method to extract miRNA from the exosomes.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1887-1889
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

MicroRNAs
Nanowires
Microchannels
Zinc oxide
Zinc Oxide
Cell culture
Antibodies
Polymethyl Methacrylate
Molecules
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Ito, S., Yasui, T., Yong, H., Yanagida, T., Rahong, S., Kanai, M., ... Baba, Y. (2013). Nanowire devices for exosomal MicroRNA extraction. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1887-1889). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.

Nanowire devices for exosomal MicroRNA extraction. / Ito, Satoru; Yasui, Takao; Yong, He; Yanagida, Takeshi; Rahong, Sakon; Kanai, Masaki; Nagashima, Kazuki; Yukawa, Hiroshi; Kaji, Noritada; Kawai, Tomoji; Baba, Yoshinobu.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1887-1889 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ito, S, Yasui, T, Yong, H, Yanagida, T, Rahong, S, Kanai, M, Nagashima, K, Yukawa, H, Kaji, N, Kawai, T & Baba, Y 2013, Nanowire devices for exosomal MicroRNA extraction. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 3, Chemical and Biological Microsystems Society, pp. 1887-1889, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Ito S, Yasui T, Yong H, Yanagida T, Rahong S, Kanai M et al. Nanowire devices for exosomal MicroRNA extraction. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1887-1889. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Ito, Satoru ; Yasui, Takao ; Yong, He ; Yanagida, Takeshi ; Rahong, Sakon ; Kanai, Masaki ; Nagashima, Kazuki ; Yukawa, Hiroshi ; Kaji, Noritada ; Kawai, Tomoji ; Baba, Yoshinobu. / Nanowire devices for exosomal MicroRNA extraction. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1887-1889 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
@inproceedings{2a988f8c5427421481bf740e8523af50,
title = "Nanowire devices for exosomal MicroRNA extraction",
abstract = "We fabricated nanowire devices, which had zinc oxide (ZnO) nanowires embedded in microchannels on PMMA substrate, to isolate exosomes from cell culture supernatant and extract MicroRNA (miRNA) from the isolated exosomes. The exosomes could be trapped by nanowires without any capturing molecules, such as antibodies against the exosomes. The exosomal miRNA could be extracted efficiently with little loss of the miRNA amount, which is comparable to that using a conventional method to extract miRNA from the exosomes.",
author = "Satoru Ito and Takao Yasui and He Yong and Takeshi Yanagida and Sakon Rahong and Masaki Kanai and Kazuki Nagashima and Hiroshi Yukawa and Noritada Kaji and Tomoji Kawai and Yoshinobu Baba",
year = "2013",
month = "1",
day = "1",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1887--1889",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",

}

TY - GEN

T1 - Nanowire devices for exosomal MicroRNA extraction

AU - Ito, Satoru

AU - Yasui, Takao

AU - Yong, He

AU - Yanagida, Takeshi

AU - Rahong, Sakon

AU - Kanai, Masaki

AU - Nagashima, Kazuki

AU - Yukawa, Hiroshi

AU - Kaji, Noritada

AU - Kawai, Tomoji

AU - Baba, Yoshinobu

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We fabricated nanowire devices, which had zinc oxide (ZnO) nanowires embedded in microchannels on PMMA substrate, to isolate exosomes from cell culture supernatant and extract MicroRNA (miRNA) from the isolated exosomes. The exosomes could be trapped by nanowires without any capturing molecules, such as antibodies against the exosomes. The exosomal miRNA could be extracted efficiently with little loss of the miRNA amount, which is comparable to that using a conventional method to extract miRNA from the exosomes.

AB - We fabricated nanowire devices, which had zinc oxide (ZnO) nanowires embedded in microchannels on PMMA substrate, to isolate exosomes from cell culture supernatant and extract MicroRNA (miRNA) from the isolated exosomes. The exosomes could be trapped by nanowires without any capturing molecules, such as antibodies against the exosomes. The exosomal miRNA could be extracted efficiently with little loss of the miRNA amount, which is comparable to that using a conventional method to extract miRNA from the exosomes.

UR - http://www.scopus.com/inward/record.url?scp=84907372425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907372425&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84907372425

SN - 9781632666246

T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

SP - 1887

EP - 1889

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -