Nanowire devices for exosomal MicroRNA extraction

Satoru Ito, Takao Yasui, He Yong, Takeshi Yanagida, Sakon Rahong, Masaki Kanai, Kazuki Nagashima, Hiroshi Yukawa, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We fabricated nanowire devices, which had zinc oxide (ZnO) nanowires embedded in microchannels on PMMA substrate, to isolate exosomes from cell culture supernatant and extract MicroRNA (miRNA) from the isolated exosomes. The exosomes could be trapped by nanowires without any capturing molecules, such as antibodies against the exosomes. The exosomal miRNA could be extracted efficiently with little loss of the miRNA amount, which is comparable to that using a conventional method to extract miRNA from the exosomes.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1887-1889
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

All Science Journal Classification (ASJC) codes

  • Bioengineering

Fingerprint Dive into the research topics of 'Nanowire devices for exosomal MicroRNA extraction'. Together they form a unique fingerprint.

Cite this