Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis

Takao Yasui, Kohei Otsuka, Masaki Takeuchi, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Sakon Rahong, Kazuki Nagashima, Toyohiro Naito, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Exploiting the method to extract DNA molecules from bacteria makes it possible to analyze bacteria in a short time. Here, we realized DNA extraction from a single bacterium via physical interactions between nanowires and bacteria; nanowire lysis. The combination of nanowire lysis and heating at 94°C could help us to extract genome DNA from bacteria, efficiently.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages320-322
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Fingerprint

Nanowires
Bacteria
DNA
Genes
Heating
Molecules

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Yasui, T., Otsuka, K., Takeuchi, M., Yanagida, T., Kaji, N., Kanai, M., ... Baba, Y. (2015). Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 320-322). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. / Yasui, Takao; Otsuka, Kohei; Takeuchi, Masaki; Yanagida, Takeshi; Kaji, Noritada; Kanai, Masaki; Rahong, Sakon; Nagashima, Kazuki; Naito, Toyohiro; Kawai, Tomoji; Baba, Yoshinobu.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 320-322 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yasui, T, Otsuka, K, Takeuchi, M, Yanagida, T, Kaji, N, Kanai, M, Rahong, S, Nagashima, K, Naito, T, Kawai, T & Baba, Y 2015, Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 320-322, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 10/25/15.
Yasui T, Otsuka K, Takeuchi M, Yanagida T, Kaji N, Kanai M et al. Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 320-322. (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Yasui, Takao ; Otsuka, Kohei ; Takeuchi, Masaki ; Yanagida, Takeshi ; Kaji, Noritada ; Kanai, Masaki ; Rahong, Sakon ; Nagashima, Kazuki ; Naito, Toyohiro ; Kawai, Tomoji ; Baba, Yoshinobu. / Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 320-322 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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