Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis

Takao Yasui, Kohei Otsuka, Masaki Takeuchi, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Sakon Rahong, Kazuki Nagashima, Toyohiro Naito, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Exploiting the method to extract DNA molecules from bacteria makes it possible to analyze bacteria in a short time. Here, we realized DNA extraction from a single bacterium via physical interactions between nanowires and bacteria; nanowire lysis. The combination of nanowire lysis and heating at 94°C could help us to extract genome DNA from bacteria, efficiently.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages320-322
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Yasui, T., Otsuka, K., Takeuchi, M., Yanagida, T., Kaji, N., Kanai, M., Rahong, S., Nagashima, K., Naito, T., Kawai, T., & Baba, Y. (2015). Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 320-322). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.