Nardonella endosymbiont in the West Indian sweet potato weevil Euscepes postfasciatus (Coleoptera: Curculionidae)

Takahiro Hosokawa, Takema Fukatsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The West Indian sweet potato weevil, Euscepes postfasciatus, is a notorious pest of the sweet potato, Ipomoea batatas. We examined the potential presence of a bacterial endosymbiont in the pest weevil. The bacterial 16S rRNA gene and groEL gene were detected by PCR from the insects. Cloning, sequencing and molecular phylogenetic analyses of the bacterial genes demonstrated that E. postfasciatus is associated with a γ -proteobacterial endosymbiont of the genus Nardonella. In situ hybridization detected the endosymbiont in the female ovaries, indicating its transovarial transmission through host generations. This study is the first to identify Nardonella from the weevil subfamily Cryptorhynchinae. The potential relevance of the endosymbiont in the biology and management of E. postfasciatus is discussed.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalApplied Entomology and Zoology
Volume45
Issue number1
DOIs
Publication statusPublished - Mar 1 2010
Externally publishedYes

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Euscepes postfasciatus
Cylas formicarius elegantulus
endosymbionts
Curculionidae
Coleoptera
pests
transovarial transmission
genes
Ipomoea batatas
sweet potatoes
in situ hybridization
molecular cloning
ribosomal RNA
Biological Sciences
insects
phylogeny

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Nardonella endosymbiont in the West Indian sweet potato weevil Euscepes postfasciatus (Coleoptera : Curculionidae). / Hosokawa, Takahiro; Fukatsu, Takema.

In: Applied Entomology and Zoology, Vol. 45, No. 1, 01.03.2010, p. 115-120.

Research output: Contribution to journalArticle

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