Nardonella endosymbionts of Japanese pest and non-pest weevils (Coleoptera

Curculionidae)

Takahiro Hosokawa, Ryuichi Koga, Kohjiro Tanaka, Minoru Moriyama, Hisashi Anbutsu, Takema Fukatsu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Many weevils are known as notorious devastating agricultural pests and generally associated with symbiotic bacteria. Here, we investigated the following pest and non-pest weevils collected in Japan for their bacterial associates: the banana stem weevil Odoiporus longicollis (Olivier); the Asiatic palm weevil Rhabdoscelus lineaticollis (Heller); the red palm weevil Rhynchophorus ferrugineus (Olivier); the Japanese giant weevil Sipalinus gigas (Fabricius); the olive weevil Pimelocerusperforatus (Roelofs); the black hard weevil Pachyrhynchus infernalis Fairmaire; and the Yonaguni hard weevil Metapocyrtus yonagunianus Chujo. Bacterial 16S rRNA gene was amplified by a polymerase chain reaction (PCR) from all the weevils, and genotyping and sequencing of the PCR products revealed that Nardonella, an ancient weevil-associated endosymbiont lineage, is the dominant bacterial associate for them. Molecular phylogenetic analyses based on bacterial 16S rRNA and groEL gene sequences showed that the weevil endosymbionts are placed within the Nardonella clade in the γ-Proteobacteria. The phylogenetic relationship of the Nardonella endosymbionts was concordant with the systematics of the weevil hosts, favoring the hypothesis of weevil-Nardonella co-speciation over evolutionary time. In situ hybridization visualized localization of the Nardonella endosymbionts in the larval bacteriome at the foregut-midgut junction in R. ferrugineus and S. gigas, and in the ovarial tips of adult females in O. longicollis. Our results highlight the general relevance of the Nardonella endosymbionts to the biology, control and management of these and other pest weevils.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalApplied Entomology and Zoology
Volume50
Issue number2
DOIs
Publication statusPublished - May 1 2015

Fingerprint

endosymbionts
Curculionidae
pests
Coleoptera
Rhynchophorus ferrugineus
Rhabdoscelus
polymerase chain reaction
ribosomal RNA
foregut
Proteobacteria
phylogeny
midgut
bananas
in situ hybridization
genotyping

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Nardonella endosymbionts of Japanese pest and non-pest weevils (Coleoptera : Curculionidae). / Hosokawa, Takahiro; Koga, Ryuichi; Tanaka, Kohjiro; Moriyama, Minoru; Anbutsu, Hisashi; Fukatsu, Takema.

In: Applied Entomology and Zoology, Vol. 50, No. 2, 01.05.2015, p. 223-229.

Research output: Contribution to journalArticle

Hosokawa, Takahiro ; Koga, Ryuichi ; Tanaka, Kohjiro ; Moriyama, Minoru ; Anbutsu, Hisashi ; Fukatsu, Takema. / Nardonella endosymbionts of Japanese pest and non-pest weevils (Coleoptera : Curculionidae). In: Applied Entomology and Zoology. 2015 ; Vol. 50, No. 2. pp. 223-229.
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