Na+-dependent fructose transport via rNaGLT1 in rat kidney

Naoshi Horiba, Satohiro Masuda, Chiharu Ohnishi, Daisuke Takeuchi, Masahiro Okuda, Ken Ichi Inui

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We found a system of Na+-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as α-methyl-D-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes.

Original languageEnglish
Pages (from-to)276-280
Number of pages5
JournalFEBS Letters
Volume546
Issue number2-3
DOIs
Publication statusPublished - Jul 10 2003

Fingerprint

Fructose
Rats
Brushes
Microvilli
Kidney
HEK293 Cells
Membranes
Facilitative Glucose Transport Proteins
Substrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Horiba, N., Masuda, S., Ohnishi, C., Takeuchi, D., Okuda, M., & Inui, K. I. (2003). Na+-dependent fructose transport via rNaGLT1 in rat kidney. FEBS Letters, 546(2-3), 276-280. https://doi.org/10.1016/S0014-5793(03)00600-8

Na+-dependent fructose transport via rNaGLT1 in rat kidney. / Horiba, Naoshi; Masuda, Satohiro; Ohnishi, Chiharu; Takeuchi, Daisuke; Okuda, Masahiro; Inui, Ken Ichi.

In: FEBS Letters, Vol. 546, No. 2-3, 10.07.2003, p. 276-280.

Research output: Contribution to journalArticle

Horiba, N, Masuda, S, Ohnishi, C, Takeuchi, D, Okuda, M & Inui, KI 2003, 'Na+-dependent fructose transport via rNaGLT1 in rat kidney', FEBS Letters, vol. 546, no. 2-3, pp. 276-280. https://doi.org/10.1016/S0014-5793(03)00600-8
Horiba N, Masuda S, Ohnishi C, Takeuchi D, Okuda M, Inui KI. Na+-dependent fructose transport via rNaGLT1 in rat kidney. FEBS Letters. 2003 Jul 10;546(2-3):276-280. https://doi.org/10.1016/S0014-5793(03)00600-8
Horiba, Naoshi ; Masuda, Satohiro ; Ohnishi, Chiharu ; Takeuchi, Daisuke ; Okuda, Masahiro ; Inui, Ken Ichi. / Na+-dependent fructose transport via rNaGLT1 in rat kidney. In: FEBS Letters. 2003 ; Vol. 546, No. 2-3. pp. 276-280.
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