Natriuretic peptide regulation of endochondral ossification: Evidence for possible roles of the C-type natriuretic peptide/guanylyl cyclase-B pathway

Akihiro Yasoda, Yoshihiro Ogawa, Michio Suda, Naohisa Tamura, Kiyoshi Mori, Yoko Sakuma, Hideki Chusho, Kohei Shiota, Kiyoshi Tanaka, Kazuwa Nakao

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

The natriuretic peptide family consists of three structurally related endogenous ligands: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). The biological actions of natriuretic peptides are thought to be mediated through the activation of two guanylyl cyclase (GC)-coupled receptor subtypes (GC-A and GC-B). In this study, we examined the effects of ANP and CNP, which are endogenous ligands for GC-A and GC-B, respectively, on bone growth using an organ culture of fetal mouse tibias, an in vitro model of endochondral ossification. CNP increased the cGMP production much more potently than ANP, thereby resulting in an increase in the total longitudinal bone length. Histological examination revealed an increase in the height of the proliferative and hypertrophic chondrocyte zones in fetal mouse tibias treated with CNP. The natriuretic peptide stimulation of bone growth, which was mimicked by 8- bromo-cGMP, was inhibited by HS-142-1, a non-peptide GC-coupled natriuretic peptide receptor antagonist. The spontaneous increase in the total longitudinal bone growth and cGMP production was also inhibited significantly by HS-142-1. CNP mRNA was expressed abundantly in fetal mouse tibias, where no significant amounts of ANP and BNP mRNAs were detected. A considerable amount of GC-B mRNA was present in fetal mouse tibias. This study suggests the physiologic significance of the CNP/GC-B pathway in the process of endochondral ossification.

Original languageEnglish
Pages (from-to)11695-11700
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number19
DOIs
Publication statusPublished - May 8 1998

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C-Type Natriuretic Peptide
Natriuretic Peptides
Guanylate Cyclase
Osteogenesis
Atrial Natriuretic Factor
Tibia
Bone Development
Bone
Brain Natriuretic Peptide
Messenger RNA
Guanylate Cyclase-Coupled Receptors
Ligands
Peptide Receptors
Organ Culture Techniques
Chondrocytes
Chemical activation
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Natriuretic peptide regulation of endochondral ossification : Evidence for possible roles of the C-type natriuretic peptide/guanylyl cyclase-B pathway. / Yasoda, Akihiro; Ogawa, Yoshihiro; Suda, Michio; Tamura, Naohisa; Mori, Kiyoshi; Sakuma, Yoko; Chusho, Hideki; Shiota, Kohei; Tanaka, Kiyoshi; Nakao, Kazuwa.

In: Journal of Biological Chemistry, Vol. 273, No. 19, 08.05.1998, p. 11695-11700.

Research output: Contribution to journalArticle

Yasoda, Akihiro ; Ogawa, Yoshihiro ; Suda, Michio ; Tamura, Naohisa ; Mori, Kiyoshi ; Sakuma, Yoko ; Chusho, Hideki ; Shiota, Kohei ; Tanaka, Kiyoshi ; Nakao, Kazuwa. / Natriuretic peptide regulation of endochondral ossification : Evidence for possible roles of the C-type natriuretic peptide/guanylyl cyclase-B pathway. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 19. pp. 11695-11700.
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AU - Mori, Kiyoshi

AU - Sakuma, Yoko

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AU - Nakao, Kazuwa

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