Natural killer T cells are involved in adipose tissues inflammation and glucose intolerance in diet-induced obese mice

Kazue Ohmura, Naoki Ishimori, Yoshinori Ohmura, Satoshi Tokuhara, Atsushi Nozawa, Shunpei Horii, Yasuhiro Andoh, Satoshi Fujii, Kazuya Iwabuchi, Kazunori Onoé, Hiroyuki Tsutsui

Research output: Contribution to journalArticlepeer-review

161 Citations (Scopus)

Abstract

BACKGROUND-: Macrophage and lymphocyte infiltration in adipose tissue may contribute to the pathogenesis of obesity-mediated metabolic disorders. Natural killer T (NKT) cells, which integrate proinflammatory cytokines, have been demonstrated in the atherosclerotic lesions and in visceral adipose tissue. OBJECTIVE-: To determine whether NKT cells are involved in glucose intolerance and adipose tissue inflammation in diet-induced obese mice. METHODS AND RESULTS-: To determine whether NKT cells are involved in the development of glucose intolerance, male β2-microglobulin knockout (KO) mice lacking NKT cells and C57BL/6J (wild-type) mice were fed with a high-fat diet (HFD) for 13 weeks. Body weight and visceral obesity were comparable between wild-type and KO mice. However, macrophage infiltration was reduced in adipose tissue and glucose intolerance was significantly ameliorated in KO mice. To further confirm that NKT cells are involved in these abnormalities, α-galactosylceramide, 0.1 μg/g body weight, which specifically activates NKT cells, was administered after 13 weeks of HFD feeding. α-Galactosylceramide significantly exacerbated glucose intolerance and macrophage infiltration as well as cytokine gene expression in adipose tissue. CONCLUSION-: NKT cells play a crucial role in the development of adipose tissue inflammation and glucose intolerance in diet-induced obesity.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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