Nbce1 and H+-atpase mRNA expression are stimulated in the mitochondria-rich cells of freshwater-acclimating Japanese eels (Anguilla japonica)

Ka Fai William Tse, Chris Kong Chu Wong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mitochondria-rich cells (MRCs) of fish gills are well known for their roles in Na+ and Cl- excretion in seawater environment. However, the level of understanding of their roles in fresh water is limited. In this study, we used the Percoll gradient technique to isolate gill MRCs from Japanese eels (Anguilla japonica Temminck and Schlegel, 1846) and measured the mRNA expression of two selected ion transporters, which were partially cloned in our laboratory: sodium bicarbonate co-transporter 1 (nbce1) and vacuolar proton pumps (H+-atpase). In addition, protein expressions of H+-ATPase and NBCe1 in whole gill were studied using Western blotting. The data indicated that both NBCe1 and H+-ATPase mRNA and protein expressions increased after seawater to freshwater transfer. This is the first report of changes in mRNA expression for these two ion transporters in MRCs of Japanese eels during seawater to freshwater transfer, confirming the roles of MRCs in osmoregulation in fresh water.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalCanadian Journal of Zoology
Volume89
Issue number4
DOIs
Publication statusPublished - Apr 1 2011
Externally publishedYes

Fingerprint

Anguilla japonica
H-transporting ATP synthase
mitochondrion
eel
mitochondria
gills
seawater
transporters
protein synthesis
cells
ions
symporters
osmoregulation
proton pump
protein
ion
sodium bicarbonate
bicarbonate
excretion
pump

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Nbce1 and H+-atpase mRNA expression are stimulated in the mitochondria-rich cells of freshwater-acclimating Japanese eels (Anguilla japonica)",
abstract = "Mitochondria-rich cells (MRCs) of fish gills are well known for their roles in Na+ and Cl- excretion in seawater environment. However, the level of understanding of their roles in fresh water is limited. In this study, we used the Percoll gradient technique to isolate gill MRCs from Japanese eels (Anguilla japonica Temminck and Schlegel, 1846) and measured the mRNA expression of two selected ion transporters, which were partially cloned in our laboratory: sodium bicarbonate co-transporter 1 (nbce1) and vacuolar proton pumps (H+-atpase). In addition, protein expressions of H+-ATPase and NBCe1 in whole gill were studied using Western blotting. The data indicated that both NBCe1 and H+-ATPase mRNA and protein expressions increased after seawater to freshwater transfer. This is the first report of changes in mRNA expression for these two ion transporters in MRCs of Japanese eels during seawater to freshwater transfer, confirming the roles of MRCs in osmoregulation in fresh water.",
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AU - Tse, Ka Fai William

AU - Wong, Chris Kong Chu

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N2 - Mitochondria-rich cells (MRCs) of fish gills are well known for their roles in Na+ and Cl- excretion in seawater environment. However, the level of understanding of their roles in fresh water is limited. In this study, we used the Percoll gradient technique to isolate gill MRCs from Japanese eels (Anguilla japonica Temminck and Schlegel, 1846) and measured the mRNA expression of two selected ion transporters, which were partially cloned in our laboratory: sodium bicarbonate co-transporter 1 (nbce1) and vacuolar proton pumps (H+-atpase). In addition, protein expressions of H+-ATPase and NBCe1 in whole gill were studied using Western blotting. The data indicated that both NBCe1 and H+-ATPase mRNA and protein expressions increased after seawater to freshwater transfer. This is the first report of changes in mRNA expression for these two ion transporters in MRCs of Japanese eels during seawater to freshwater transfer, confirming the roles of MRCs in osmoregulation in fresh water.

AB - Mitochondria-rich cells (MRCs) of fish gills are well known for their roles in Na+ and Cl- excretion in seawater environment. However, the level of understanding of their roles in fresh water is limited. In this study, we used the Percoll gradient technique to isolate gill MRCs from Japanese eels (Anguilla japonica Temminck and Schlegel, 1846) and measured the mRNA expression of two selected ion transporters, which were partially cloned in our laboratory: sodium bicarbonate co-transporter 1 (nbce1) and vacuolar proton pumps (H+-atpase). In addition, protein expressions of H+-ATPase and NBCe1 in whole gill were studied using Western blotting. The data indicated that both NBCe1 and H+-ATPase mRNA and protein expressions increased after seawater to freshwater transfer. This is the first report of changes in mRNA expression for these two ion transporters in MRCs of Japanese eels during seawater to freshwater transfer, confirming the roles of MRCs in osmoregulation in fresh water.

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