Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels

Yoichi Sasaki, Mio Oshikawa, Pankaj Bharmoria, Hironori Kouno, Akiko Hayashi-Takagi, Moritoshi Sato, Itsuki Ajioka, Nobuhiro Yanai, Nobuo Kimizuka

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.

Original languageEnglish
Pages (from-to)17827-17833
Number of pages7
JournalAngewandte Chemie - International Edition
Volume58
Issue number49
DOIs
Publication statusPublished - Dec 2 2019

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Hydrogels
Photons
Genes
Infrared radiation
UCON 50-HB-5100
Hydrogel
Poloxamer
Micelles
Chromophores
Neurons
Heat treatment
Tissue
Oxygen
Nanoparticles

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels. / Sasaki, Yoichi; Oshikawa, Mio; Bharmoria, Pankaj; Kouno, Hironori; Hayashi-Takagi, Akiko; Sato, Moritoshi; Ajioka, Itsuki; Yanai, Nobuhiro; Kimizuka, Nobuo.

In: Angewandte Chemie - International Edition, Vol. 58, No. 49, 02.12.2019, p. 17827-17833.

Research output: Contribution to journalReview article

Sasaki, Y, Oshikawa, M, Bharmoria, P, Kouno, H, Hayashi-Takagi, A, Sato, M, Ajioka, I, Yanai, N & Kimizuka, N 2019, 'Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels', Angewandte Chemie - International Edition, vol. 58, no. 49, pp. 17827-17833. https://doi.org/10.1002/anie.201911025
Sasaki Y, Oshikawa M, Bharmoria P, Kouno H, Hayashi-Takagi A, Sato M et al. Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels. Angewandte Chemie - International Edition. 2019 Dec 2;58(49):17827-17833. https://doi.org/10.1002/anie.201911025
Sasaki, Yoichi ; Oshikawa, Mio ; Bharmoria, Pankaj ; Kouno, Hironori ; Hayashi-Takagi, Akiko ; Sato, Moritoshi ; Ajioka, Itsuki ; Yanai, Nobuhiro ; Kimizuka, Nobuo. / Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels. In: Angewandte Chemie - International Edition. 2019 ; Vol. 58, No. 49. pp. 17827-17833.
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