TY - JOUR
T1 - Near-Infrared Optogenetic Genome Engineering Based on Photon-Upconversion Hydrogels
AU - Sasaki, Yoichi
AU - Oshikawa, Mio
AU - Bharmoria, Pankaj
AU - Kouno, Hironori
AU - Hayashi-Takagi, Akiko
AU - Sato, Moritoshi
AU - Ajioka, Itsuki
AU - Yanai, Nobuhiro
AU - Kimizuka, Nobuo
N1 - Funding Information:
This work was partly supported by JSPS KAKENHI grant number JP25220805, JP17H04799, JP16H06513, JP16H00844, PRESTO program on “Molecular Technology and Creation of New Functions” from JST (JPMJPR14KE, JPMJPR14K1), the Izumi Science and Technology Foundation, and the Asahi Glass Foundation. P.B. acknowledges JSPS postdoctoral fellowships for foreign researchers.
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/12/2
Y1 - 2019/12/2
N2 - Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.
AB - Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.
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U2 - 10.1002/anie.201911025
DO - 10.1002/anie.201911025
M3 - Review article
C2 - 31544993
AN - SCOPUS:85074480862
SN - 1433-7851
VL - 58
SP - 17827
EP - 17833
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 49
ER -