Near-IR laser-triggered target cell collection using a carbon nanotube-based cell-cultured substrate

Takao Sada, Tsuyohiko Fujigaya, Yasuro Niidome, Kohji Nakazawa, Naotoshi Nakashima

    Research output: Contribution to journalArticlepeer-review

    32 Citations (Scopus)

    Abstract

    Unique near-IR optical properties of single-walled carbon nanotube (SWNTs) are of interest in many biological applications. Here we describe the selective cell detachment and collection from an SWNT-coated cell-culture dish triggered by near-IR pulse laser irradiation. First, HeLa cells were cultured on an SWNT-coated dish prepared by a spraying of an aqueous SWNT dispersion on a glass dish. The SWNT-coated dish was found to show a good cell adhesion behavior as well as a cellular proliferation rate similar to a conventional glass dish. We discovered, by near-IR pulse laser irradiation (at the laser power over 25 mW) to the cell under optical microscopic observation, a quick single-cell detachment from the SWNT-coated surface. Shockwave generation from the irradiated SWNTs is expected to play an important role for the cell detachment. Moreover, we have succeeded in catapulting the target single cell from the cultured medium when the depth of the medium was below 150 μm and the laser power was stronger than 40 mW. The captured cell maintained its original shape. The retention of the genetic information of the cell was confirmed by the polymerase chain reaction (PCR) technique. A target single-cell collection from a culture medium under optical microscopic observation is significant in wide fields of single-cell studies in biological areas.

    Original languageEnglish
    Pages (from-to)4414-4421
    Number of pages8
    JournalACS nano
    Volume5
    Issue number6
    DOIs
    Publication statusPublished - Jun 28 2011

    All Science Journal Classification (ASJC) codes

    • Materials Science(all)
    • Engineering(all)
    • Physics and Astronomy(all)

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