Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells

Paerwen Paerhati, Akira Ito, Kantaro Yoshioka, Kaori Iwamoto, Sho Fujiwara, Masanobu Horie, Kawabe Yoshinori, Masamichi Kamihira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered PA6 feeder cells will enhance the performance of SDIA by facilitating cell–cell interactions. Consequently, we created cadherin gene-engineered PA6 cells. Efficiency of neural differentiation from mouse iPS cells on PA6 feeder cells overexpressing E-cadherin gene (46%) or N-cadherin gene (27%) was significantly higher compared with parental PA6 feeder cells (19%). In addition, efficiency of motor neuron differentiation from mouse iPS cells on cadherin-gene engineered feeder cells (E-cadherin, 7.4%; N-cadherin, 11%) was significantly higher compared with parental PA6 feeder cells (4.1%). Altogether, these results indicate that cadherin gene-engineered feeder cells are a potent tool for promoting neural differentiation of pluripotent stem cells.

Original languageEnglish
Pages (from-to)633-640
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume127
Issue number5
DOIs
Publication statusPublished - May 1 2019

Fingerprint

Feeder Cells
Induced Pluripotent Stem Cells
Cadherins
Stem cells
Genes
Stromal Cells
Pluripotent Stem Cells
Nerve Regeneration
Neurons
Motor Neurons

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells. / Paerhati, Paerwen; Ito, Akira; Yoshioka, Kantaro; Iwamoto, Kaori; Fujiwara, Sho; Horie, Masanobu; Yoshinori, Kawabe; Kamihira, Masamichi.

In: Journal of Bioscience and Bioengineering, Vol. 127, No. 5, 01.05.2019, p. 633-640.

Research output: Contribution to journalArticle

Paerhati, Paerwen ; Ito, Akira ; Yoshioka, Kantaro ; Iwamoto, Kaori ; Fujiwara, Sho ; Horie, Masanobu ; Yoshinori, Kawabe ; Kamihira, Masamichi. / Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells. In: Journal of Bioscience and Bioengineering. 2019 ; Vol. 127, No. 5. pp. 633-640.
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