Neuroepithelial progenitors undergo LGN-dependent planar divisions to maintain self-renewability during mammalian neurogenesis

Daijiro Konno, Go Shioi, Atsunori Shitamukai, Asako Mori, Hiroshi Kiyonari, Takaki Miyata, Fumio Matsuzaki

Research output: Contribution to journalArticle

321 Citations (Scopus)

Abstract

During mammalian development, neuroepithelial cells function as mitotic progenitors, which self-renew and generate neurons. Although spindle orientation is important for such polarized cells to undergo symmetric or asymmetric divisions, its role in mammalian neurogenesis remains unclear. Here we show that control of spindle orientation is essential in maintaining the population of neuroepithelial cells, but dispensable for the decision to either proliferate or differentiate. Knocking out LGN, (the G protein regulator), randomized the orientation of normally planar neuroepithelial divisions. The resultant loss of the apical membrane from daughter cells frequently converted them into abnormally localized progenitors without affecting neuronal production rate. Furthermore, overexpression of Inscuteable to induce vertical neuroepithelial divisions shifted the fate of daughter cells. Our results suggest that planar mitosis ensures the self-renewal of neuroepithelial progenitors by one daughter inheriting both apical and basal compartments during neurogenesis.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalNature Cell Biology
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

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Neuroepithelial Cells
Neurogenesis
GTP-Binding Proteins
Mitosis
Cell Membrane
Neurons
Population

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Neuroepithelial progenitors undergo LGN-dependent planar divisions to maintain self-renewability during mammalian neurogenesis. / Konno, Daijiro; Shioi, Go; Shitamukai, Atsunori; Mori, Asako; Kiyonari, Hiroshi; Miyata, Takaki; Matsuzaki, Fumio.

In: Nature Cell Biology, Vol. 10, No. 1, 01.01.2008, p. 93-101.

Research output: Contribution to journalArticle

Konno, Daijiro ; Shioi, Go ; Shitamukai, Atsunori ; Mori, Asako ; Kiyonari, Hiroshi ; Miyata, Takaki ; Matsuzaki, Fumio. / Neuroepithelial progenitors undergo LGN-dependent planar divisions to maintain self-renewability during mammalian neurogenesis. In: Nature Cell Biology. 2008 ; Vol. 10, No. 1. pp. 93-101.
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