Neuron-specific γ-enolase derived from human glioma

Masaharu Kuramitsu, Hiroki Sawa, Iwao Takeshita, Toru Iwaki, Kanefusa Kato

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Neuron-specific γ-enolase in human neurogenic tumors, including gliomas, transplanted gliomas, and permanent human glioma cell lines, was studied quantitatively, using newly established enzyme immunoassay methods, together with immunostaining of the tissue and cell preparations. A significantly high level of γ-enolase was found in some glioblastomas, astrocytomas and oligodendrogliomas as well as medulloblastomas. Glioblastomas transplanted into mice and cultured cell lines derived from the same origins, as well as the permanent human glioma cell lines, also contained γ-enolase, although the contents were low compared with findings in the original tumor tissues. Immunohistochemically, γ-enolase stained intensely in the glioblastomatous cells. Serum γ-enolase concentrations in some patients with gliomas and those of all the transplanted mice were enhanced. The serum γ-enolase levels in the mice correlated well with size of the transplanted tumor tissues. These results indicate that neuron-specific γ-enolase is produced in some neurogenic tumors of nonneuronal origin, therefore, serum γ-enolase may be a useful biomarker for monitoring the extent of disease in patients with gliomas.

Original languageEnglish
Pages (from-to)89-105
Number of pages17
JournalNeurochemical Pathology
Volume4
Issue number2
DOIs
Publication statusPublished - Apr 1 1986

Fingerprint

Phosphopyruvate Hydratase
Glioma
Glioblastoma
Cell Line
Neoplasms
Serum
Oligodendroglioma
Medulloblastoma
Astrocytoma
Immunoenzyme Techniques
Cultured Cells
Biomarkers

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology

Cite this

Neuron-specific γ-enolase derived from human glioma. / Kuramitsu, Masaharu; Sawa, Hiroki; Takeshita, Iwao; Iwaki, Toru; Kato, Kanefusa.

In: Neurochemical Pathology, Vol. 4, No. 2, 01.04.1986, p. 89-105.

Research output: Contribution to journalArticle

Kuramitsu, M, Sawa, H, Takeshita, I, Iwaki, T & Kato, K 1986, 'Neuron-specific γ-enolase derived from human glioma', Neurochemical Pathology, vol. 4, no. 2, pp. 89-105. https://doi.org/10.1007/BF03160188
Kuramitsu, Masaharu ; Sawa, Hiroki ; Takeshita, Iwao ; Iwaki, Toru ; Kato, Kanefusa. / Neuron-specific γ-enolase derived from human glioma. In: Neurochemical Pathology. 1986 ; Vol. 4, No. 2. pp. 89-105.
@article{8f896857d5d04ff28010c964b7b912a0,
title = "Neuron-specific γ-enolase derived from human glioma",
abstract = "Neuron-specific γ-enolase in human neurogenic tumors, including gliomas, transplanted gliomas, and permanent human glioma cell lines, was studied quantitatively, using newly established enzyme immunoassay methods, together with immunostaining of the tissue and cell preparations. A significantly high level of γ-enolase was found in some glioblastomas, astrocytomas and oligodendrogliomas as well as medulloblastomas. Glioblastomas transplanted into mice and cultured cell lines derived from the same origins, as well as the permanent human glioma cell lines, also contained γ-enolase, although the contents were low compared with findings in the original tumor tissues. Immunohistochemically, γ-enolase stained intensely in the glioblastomatous cells. Serum γ-enolase concentrations in some patients with gliomas and those of all the transplanted mice were enhanced. The serum γ-enolase levels in the mice correlated well with size of the transplanted tumor tissues. These results indicate that neuron-specific γ-enolase is produced in some neurogenic tumors of nonneuronal origin, therefore, serum γ-enolase may be a useful biomarker for monitoring the extent of disease in patients with gliomas.",
author = "Masaharu Kuramitsu and Hiroki Sawa and Iwao Takeshita and Toru Iwaki and Kanefusa Kato",
year = "1986",
month = "4",
day = "1",
doi = "10.1007/BF03160188",
language = "English",
volume = "4",
pages = "89--105",
journal = "Journal of Molecular Neuroscience",
issn = "0895-8696",
publisher = "Humana Press",
number = "2",

}

TY - JOUR

T1 - Neuron-specific γ-enolase derived from human glioma

AU - Kuramitsu, Masaharu

AU - Sawa, Hiroki

AU - Takeshita, Iwao

AU - Iwaki, Toru

AU - Kato, Kanefusa

PY - 1986/4/1

Y1 - 1986/4/1

N2 - Neuron-specific γ-enolase in human neurogenic tumors, including gliomas, transplanted gliomas, and permanent human glioma cell lines, was studied quantitatively, using newly established enzyme immunoassay methods, together with immunostaining of the tissue and cell preparations. A significantly high level of γ-enolase was found in some glioblastomas, astrocytomas and oligodendrogliomas as well as medulloblastomas. Glioblastomas transplanted into mice and cultured cell lines derived from the same origins, as well as the permanent human glioma cell lines, also contained γ-enolase, although the contents were low compared with findings in the original tumor tissues. Immunohistochemically, γ-enolase stained intensely in the glioblastomatous cells. Serum γ-enolase concentrations in some patients with gliomas and those of all the transplanted mice were enhanced. The serum γ-enolase levels in the mice correlated well with size of the transplanted tumor tissues. These results indicate that neuron-specific γ-enolase is produced in some neurogenic tumors of nonneuronal origin, therefore, serum γ-enolase may be a useful biomarker for monitoring the extent of disease in patients with gliomas.

AB - Neuron-specific γ-enolase in human neurogenic tumors, including gliomas, transplanted gliomas, and permanent human glioma cell lines, was studied quantitatively, using newly established enzyme immunoassay methods, together with immunostaining of the tissue and cell preparations. A significantly high level of γ-enolase was found in some glioblastomas, astrocytomas and oligodendrogliomas as well as medulloblastomas. Glioblastomas transplanted into mice and cultured cell lines derived from the same origins, as well as the permanent human glioma cell lines, also contained γ-enolase, although the contents were low compared with findings in the original tumor tissues. Immunohistochemically, γ-enolase stained intensely in the glioblastomatous cells. Serum γ-enolase concentrations in some patients with gliomas and those of all the transplanted mice were enhanced. The serum γ-enolase levels in the mice correlated well with size of the transplanted tumor tissues. These results indicate that neuron-specific γ-enolase is produced in some neurogenic tumors of nonneuronal origin, therefore, serum γ-enolase may be a useful biomarker for monitoring the extent of disease in patients with gliomas.

UR - http://www.scopus.com/inward/record.url?scp=0022694707&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022694707&partnerID=8YFLogxK

U2 - 10.1007/BF03160188

DO - 10.1007/BF03160188

M3 - Article

C2 - 3014401

AN - SCOPUS:0022694707

VL - 4

SP - 89

EP - 105

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

SN - 0895-8696

IS - 2

ER -