Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3

Makoto Ohno, Jun Kohyama, Masakazu Namihira, Tsukasa Sanosaka, Jun A. Takahashi, Nobuo Hashimoto, Kinichi Nakashima

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Neuropoietin (NP) is a recently identified member of the interleukin (IL)-6 family of cytokines, which share glycoprotein 130 (gp130) as a signal-transducing receptor component, and is highly expressed in embryonic brain. In this study, we show that NP has the potential to induce neuroepithelial cells to differentiate into astrocytes. NP stimulation leads to promoter activation of the gene for an astrocyte marker, glial fibrillary acidic protein (GFAP), which is clearly inhibited by either expression of a dominant-negative form of a transcription factor, signal transducer and activator of transcription 3 (STAT3) or by a nucleotide-substitution in the STAT3-binding element within the gene promoter. We further show that NP induces binding of endogenous STAT3 to its cognate sequence within the gfap gene promoter in neuroepithelial cells. Moreover, like the other IL-6 cytokine family members, NP promotes astrocyte differentiation in a synergistic manner with bone-morphogenetic protein (BMP)-2. Taken together, our data indicate that NP can be considered as a new astrocyte-inducing cytokine in the developing brain.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalCytokine
Volume36
Issue number1-2
DOIs
Publication statusPublished - Oct 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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