Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting

Eri Sumiya, Yukiko Ogino, Kenji Toyota, Hitoshi Miyakawa, Shinichi Miyagawa, Taisen Iguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Embryo development in arthropods is accompanied by a series of moltings. A cladoceran crustacean Daphnia magna molts three times before reaching first instar neonate during embryogenesis. Previous studies argued ecdysteroids might regulate D. magna embryogenesis. However, no direct evidence between innate ecdysteroids fluctuation and functions has been forthcoming. Recently, we identified genes involved in ecdysteroid synthesis called, neverland (neverland1 and neverland 2) and shade and in the ecdysteroid degradation (Cyp18a1). To understand the physiological roles of ecdysteroids in D. magna embryos, we performed expression and functional analyzes of those genes. Examining innate ecdysteroids titer during embryogenesis showed two surges of ecdysteroids titer at 41 and 61 h after oviposition. The first and second embryonic moltings occurred at each ecdysteroid surge. Expression of neverland1 and shade began to increase before the first peak in ecdysteroid. Knockdown of neverland1 or shade by RNAi technique caused defects in embryonic moltings and subsequent development. The ecdysteroids titer seemingly decreased in nvd1-knowckdown embryos. Knockdown of Cyp18a1 resulted in early embryonic lethality before the first molting. Our in situ hybridization analysis revealed that nvd1 was prominently expressed in embryonic gut epithelium suggesting the site for an initial step of ecdysteroidgenesis, a conversion of cholesterol to 7-dehydrocholesterol and possibly for ecdysone production. Taken together, de novo ecdysteroid synthesis by nvd1 in the gut epithelial cells stimulates molting, which is indispensable for D. magna embryo development. These findings identify neverland as a possible target for chemicals, including various pesticides that are known to disrupt molting, development and reproduction.

Original languageEnglish
Pages (from-to)1476-1485
Number of pages10
JournalJournal of Applied Toxicology
Volume36
Issue number11
DOIs
Publication statusPublished - Nov 1 2016
Externally publishedYes

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Cladocera
Ecdysteroids
Daphnia
Molting
Water
Embryonic Development
Embryonic Structures
Genes
Ecdysone
Oviposition
Arthropods
RNA Interference
Pesticides
In Situ Hybridization
Reproduction

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting. / Sumiya, Eri; Ogino, Yukiko; Toyota, Kenji; Miyakawa, Hitoshi; Miyagawa, Shinichi; Iguchi, Taisen.

In: Journal of Applied Toxicology, Vol. 36, No. 11, 01.11.2016, p. 1476-1485.

Research output: Contribution to journalArticle

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