New bile pigment excreted by a Chlamydomonas reinhardtii mutant: A possible breakdown catabolite of chlorophylla

Michio Doi, S. Shima, T. Egashira, K. Nakamura, S. Okayama

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A chlorophyll b-less mutant of Chlamydomonas reinhardtii excreted new red pigments into the medium accompanying degreening of cells during growth. Accumulation of red pigments in the medium and disappearance of chlorophyll a in algal cells were closely related. A major component (P535) was purified by reversed-phase chromatography, and showed absorption maxima at 535, 385, and 275 nm with shoulders at 570, 500, and 320 nm. Analysis of degradation products by chromic oxidation, together with spectroscopic properties and molecular-mass determination, indicated that P535 bore a structural resemblance to bile pigments characterized by an open tetrapyrrole. It is suggested that the red pigments are probably derived from chlorophyll a.

Original languageEnglish
Pages (from-to)504-508
Number of pages5
JournalJournal of Plant Physiology
Volume150
Issue number5
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

bile pigments
Bile Pigments
Chlamydomonas reinhardtii
pigments
Tetrapyrroles
metabolites
chlorophyll
mutants
Reverse-Phase Chromatography
degreening
shoulders
cell growth
chromatography
Growth
oxidation
molecular weight
degradation
chlorophyll a
cells

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

New bile pigment excreted by a Chlamydomonas reinhardtii mutant : A possible breakdown catabolite of chlorophylla. / Doi, Michio; Shima, S.; Egashira, T.; Nakamura, K.; Okayama, S.

In: Journal of Plant Physiology, Vol. 150, No. 5, 01.01.1997, p. 504-508.

Research output: Contribution to journalArticle

Doi, Michio ; Shima, S. ; Egashira, T. ; Nakamura, K. ; Okayama, S. / New bile pigment excreted by a Chlamydomonas reinhardtii mutant : A possible breakdown catabolite of chlorophylla. In: Journal of Plant Physiology. 1997 ; Vol. 150, No. 5. pp. 504-508.
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