New detection method of biological targets based on HTS SQUID and magnetic marker

M. Ura, Y. Ueoka, K. Noguchi, K. Nakamura, T. Sasayama, T. Yoshida, K. Enpuku

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We developed improved methods of liquid-phase detection of biological targets using HTS SQUID and Brownian relaxation of magnetic markers. In a real sample, the aggregation and precipitation of free markers deteriorate their Brownian relaxation and cause so-called blank signals. In order to solve this problem, we developed two methods. First, we introduced a reaction field Bre during the binding reaction between the markers and targets. Using this method, we could obtain a strong signal from the bound markers without increasing the aggregation of the free markers. Next, we introduced a fieldreversal method in the measurement procedure in order to differentiate the signal from the markers in suspension from that from the precipitated markers. Using this procedure, we could eliminate the signal from the precipitated markers. Then, we detected biotin molecules by using these methods. The biotins were immobilized on the surfaces of large polymer beads with diameters of 3.3 μm and were detected with streptavidinconjugated magnetic markers. The minimum detectable molecular number concentration was 1.8 × 10-19mol/ml, indicating the high sensitivity of the present method.

Original languageEnglish
Title of host publication2015 15th International Superconductive Electronics Conference, ISEC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781467383486
DOIs
Publication statusPublished - Jan 14 2016
Event15th International Superconductive Electronics Conference, ISEC 2015 - Nagoya, Japan
Duration: Jul 6 2015Jul 9 2015

Publication series

Name2015 15th International Superconductive Electronics Conference, ISEC 2015

Other

Other15th International Superconductive Electronics Conference, ISEC 2015
CountryJapan
CityNagoya
Period7/6/157/9/15

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

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    Ura, M., Ueoka, Y., Noguchi, K., Nakamura, K., Sasayama, T., Yoshida, T., & Enpuku, K. (2016). New detection method of biological targets based on HTS SQUID and magnetic marker. In 2015 15th International Superconductive Electronics Conference, ISEC 2015 [7383474] (2015 15th International Superconductive Electronics Conference, ISEC 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ISEC.2015.7383474