New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus

T. Sekiguchi, H. Sasaki, Y. Kurihara, S. Watanabe, D. Moriyama, N. Kurose, R. Matsuki, K. Yamazaki, M. Saeki

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We developed novel species and sex determination methods for three Japanese mustelid species. We used DDX3Y to determine sex and generated a primer set to amplify both DDX3X and DDX3Y DNA in Mustela itatsi, M. sibirica and Martes melampus. To determine species and sex simultaneously, we generated fluorescence-labelled primers that give different fragment lengths at D-loop, DDX3X and DDX3Y of these three species using a DNA sequencer.

Original languageEnglish
Pages (from-to)1089-1091
Number of pages3
JournalMolecular Ecology Resources
Issue number6
Publication statusPublished - Nov 1 2010


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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