New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus

Takeshi Sekiguchi, H. Sasaki, Y. Kurihara, S. Watanabe, D. Moriyama, N. Kurose, R. Matsuki, K. Yamazaki, M. Saeki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed novel species and sex determination methods for three Japanese mustelid species. We used DDX3Y to determine sex and generated a primer set to amplify both DDX3X and DDX3Y DNA in Mustela itatsi, M. sibirica and Martes melampus. To determine species and sex simultaneously, we generated fluorescence-labelled primers that give different fragment lengths at D-loop, DDX3X and DDX3Y of these three species using a DNA sequencer.

Original languageEnglish
Pages (from-to)1089-1091
Number of pages3
JournalMolecular Ecology Resources
Volume10
Issue number6
DOIs
Publication statusPublished - Nov 1 2010

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mustelid
Mustelidae
Mustela
sex determination
Martes
gender
DNA
Fluorescence
methodology
fluorescence
Mustela sibirica
method

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus. / Sekiguchi, Takeshi; Sasaki, H.; Kurihara, Y.; Watanabe, S.; Moriyama, D.; Kurose, N.; Matsuki, R.; Yamazaki, K.; Saeki, M.

In: Molecular Ecology Resources, Vol. 10, No. 6, 01.11.2010, p. 1089-1091.

Research output: Contribution to journalArticle

Sekiguchi, Takeshi ; Sasaki, H. ; Kurihara, Y. ; Watanabe, S. ; Moriyama, D. ; Kurose, N. ; Matsuki, R. ; Yamazaki, K. ; Saeki, M. / New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus. In: Molecular Ecology Resources. 2010 ; Vol. 10, No. 6. pp. 1089-1091.
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