TY - JOUR
T1 - New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus
AU - Sekiguchi, T.
AU - Sasaki, H.
AU - Kurihara, Y.
AU - Watanabe, S.
AU - Moriyama, D.
AU - Kurose, N.
AU - Matsuki, R.
AU - Yamazaki, K.
AU - Saeki, M.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/11
Y1 - 2010/11
N2 - We developed novel species and sex determination methods for three Japanese mustelid species. We used DDX3Y to determine sex and generated a primer set to amplify both DDX3X and DDX3Y DNA in Mustela itatsi, M. sibirica and Martes melampus. To determine species and sex simultaneously, we generated fluorescence-labelled primers that give different fragment lengths at D-loop, DDX3X and DDX3Y of these three species using a DNA sequencer.
AB - We developed novel species and sex determination methods for three Japanese mustelid species. We used DDX3Y to determine sex and generated a primer set to amplify both DDX3X and DDX3Y DNA in Mustela itatsi, M. sibirica and Martes melampus. To determine species and sex simultaneously, we generated fluorescence-labelled primers that give different fragment lengths at D-loop, DDX3X and DDX3Y of these three species using a DNA sequencer.
UR - http://www.scopus.com/inward/record.url?scp=78650426189&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650426189&partnerID=8YFLogxK
U2 - 10.1111/j.1755-0998.2010.02842.x
DO - 10.1111/j.1755-0998.2010.02842.x
M3 - Article
C2 - 21565122
AN - SCOPUS:78650426189
SN - 1755-098X
VL - 10
SP - 1089
EP - 1091
JO - Molecular Ecology Notes
JF - Molecular Ecology Notes
IS - 6
ER -