New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111

Hidemasa Izumiya, Yingxin Pei, Jun Terajima, Makoto Ohnishi, Tetsuya Hayashi, Sunao Iyoda, Haruo Watanabe

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC), a food- and waterborne pathogen, causes diarrhea, hemorrhagic colitis, and life-threatening HUS. MLVA is a newly developed and widely accepted genotyping tool. An MLVA system for EHEC O157 involving nine genomic loci has already been established. However, the present study revealed that the above-mentioned MLVA system cannot analyze EHEC O26 and O111 isolates-the second and third most dominant EHEC serogroups in Japan, respectively. Therefore, with several modifications to the O157 system and the use of nine additional loci, we developed an expanded MLVA system applicable to EHEC O26, O111, and O157. Our MLVA system had a relatively high resolution power for each of the three serogroups: Simpson's index of diversity was 0.991 (95% CI = 0.989-0.993), 0.988 (95% CI, 0.986-0.990), and 0.986 (95% CI, 0.979-0.993) for O26, O111, and O157, respectively. This systemalso detected outbreak-related isolates; the isolates collected during each of the 12 O26 and O111 outbreaks formed unique clusters, and most of the repeat copy numbers among the isolates collected during the sameoutbreak exhibited no or single-locus variations. These resultswere comparable to those of cluster analyses based on PFGE profiles. Therefore, our system can complement PFGE analysis-the current golden method. Because EHEC strains of three major serogroups can be rapidly analyzed on a single platformwith our expanded MLVA system, this systemcould be widely used in molecular epidemiological studies of EHEC infections.

Original languageEnglish
Pages (from-to)569-577
Number of pages9
JournalMICROBIOLOGY and IMMUNOLOGY
Volume54
Issue number10
DOIs
Publication statusPublished - Oct 1 2010

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Enterohemorrhagic Escherichia coli
Minisatellite Repeats
Escherichia coli O157
Disease Outbreaks
Escherichia coli Infections
Colitis
Serogroup
Cluster Analysis
Epidemiologic Studies
Diarrhea
Japan
Food

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups : O157, O26, and O111. / Izumiya, Hidemasa; Pei, Yingxin; Terajima, Jun; Ohnishi, Makoto; Hayashi, Tetsuya; Iyoda, Sunao; Watanabe, Haruo.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 54, No. 10, 01.10.2010, p. 569-577.

Research output: Contribution to journalArticle

Izumiya, Hidemasa ; Pei, Yingxin ; Terajima, Jun ; Ohnishi, Makoto ; Hayashi, Tetsuya ; Iyoda, Sunao ; Watanabe, Haruo. / New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups : O157, O26, and O111. In: MICROBIOLOGY and IMMUNOLOGY. 2010 ; Vol. 54, No. 10. pp. 569-577.
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abstract = "Enterohemorrhagic Escherichia coli (EHEC), a food- and waterborne pathogen, causes diarrhea, hemorrhagic colitis, and life-threatening HUS. MLVA is a newly developed and widely accepted genotyping tool. An MLVA system for EHEC O157 involving nine genomic loci has already been established. However, the present study revealed that the above-mentioned MLVA system cannot analyze EHEC O26 and O111 isolates-the second and third most dominant EHEC serogroups in Japan, respectively. Therefore, with several modifications to the O157 system and the use of nine additional loci, we developed an expanded MLVA system applicable to EHEC O26, O111, and O157. Our MLVA system had a relatively high resolution power for each of the three serogroups: Simpson's index of diversity was 0.991 (95{\%} CI = 0.989-0.993), 0.988 (95{\%} CI, 0.986-0.990), and 0.986 (95{\%} CI, 0.979-0.993) for O26, O111, and O157, respectively. This systemalso detected outbreak-related isolates; the isolates collected during each of the 12 O26 and O111 outbreaks formed unique clusters, and most of the repeat copy numbers among the isolates collected during the sameoutbreak exhibited no or single-locus variations. These resultswere comparable to those of cluster analyses based on PFGE profiles. Therefore, our system can complement PFGE analysis-the current golden method. Because EHEC strains of three major serogroups can be rapidly analyzed on a single platformwith our expanded MLVA system, this systemcould be widely used in molecular epidemiological studies of EHEC infections.",
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