Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals

Keita Tateishi, Kanji Okumoto, Nobuyuki Shimozawa, Toshiro Tsukamoto, Takashi Osumi, Yasuyuki Suzuki, Naomi Kondo, Ichiro Okano, Yukio Fujiki

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Abstract

We isolated peroxisome biogenesis mutants from Chinese hamster ovary (CHO) cells, using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and wild-type CHO-K1 cells that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1). Three mutant cell clones, ZP110, ZP111, and ZP114, showed cytosolic localization of catalase, thereby indicating a defect in peroxisome biogenesis, whereas ZP112 and ZP113 contained fewer but larger catalase-positive particles. Mutant ZP115 displayed an aberrant, tubular structure immunoreactive to anti-catalase antibody. Mutants lacking morphologically recognizable peroxisomes also showed the typical peroxisome assembly-defective phenotype such as severe loss of catalase latency and resistance to 12-(1'-pyrene)dodecanoic acid (P12)/UV treatment. ZP110 and ZP111, and ZP114 were found to belong to two novel complementation groups, respectively, by complementation group analysis with cDNA transfection and cell fusion. Cell fusion with fibroblasts from patients with peroxisome biogenesis disorders such as Zellweger syndrome revealed that ZP110 and ZP114 could not be classified to any of human complementation groups. Thus, ZP110/ZP111 and ZP114 are the first, two peroxisome-deficient cell mutants of newly identified complementation groups distinct from the ten mammalian groups previously characterized.

Original languageEnglish
Pages (from-to)352-359
Number of pages8
JournalEuropean Journal of Cell Biology
Volume73
Issue number4
Publication statusPublished - Jan 1 1997

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Peroxisomes
Cricetulus
Mammals
Ovary
Catalase
Cell Fusion
Complementary DNA
Zellweger Syndrome
Transfection
Anti-Idiotypic Antibodies
Clone Cells
Fibroblasts
Phenotype

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals. / Tateishi, Keita; Okumoto, Kanji; Shimozawa, Nobuyuki; Tsukamoto, Toshiro; Osumi, Takashi; Suzuki, Yasuyuki; Kondo, Naomi; Okano, Ichiro; Fujiki, Yukio.

In: European Journal of Cell Biology, Vol. 73, No. 4, 01.01.1997, p. 352-359.

Research output: Contribution to journalArticle

Tateishi, K, Okumoto, K, Shimozawa, N, Tsukamoto, T, Osumi, T, Suzuki, Y, Kondo, N, Okano, I & Fujiki, Y 1997, 'Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals', European Journal of Cell Biology, vol. 73, no. 4, pp. 352-359.
Tateishi, Keita ; Okumoto, Kanji ; Shimozawa, Nobuyuki ; Tsukamoto, Toshiro ; Osumi, Takashi ; Suzuki, Yasuyuki ; Kondo, Naomi ; Okano, Ichiro ; Fujiki, Yukio. / Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals. In: European Journal of Cell Biology. 1997 ; Vol. 73, No. 4. pp. 352-359.
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