NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Dawei Yan; Vanathy Easwaran; Vivian Chau; Masanori Okamoto; Matthew Ierullo; Mitsuhiro Kimura; Akira Endo; Ryoichi Yano; Asher Pasha; Yunchen Gong; Yong Mei Bi; Nicolas Provart; David Guttman; Anne Krapp; Steven J. Rothstein; Eiji Nambara

doi:10.1038/ncomms13179

NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Dawei Yan, Vanathy Easwaran, Vivian Chau, Masanori Okamoto, Matthew Ierullo, Mitsuhiro Kimura, Akira Endo, Ryoichi Yano, Asher Pasha, Yunchen Gong, Yong Mei Bi, Nicolas Provart, David Guttman, Anne Krapp, Steven J. Rothstein, Eiji Nambara

Research output: Contribution to journal › Article › peer-review

108 Citations (Scopus)

Abstract

Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

Original language	English
Article number	13179
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms13179
Publication status	Published - Oct 12 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis",

abstract = "Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.",

author = "Dawei Yan and Vanathy Easwaran and Vivian Chau and Masanori Okamoto and Matthew Ierullo and Mitsuhiro Kimura and Akira Endo and Ryoichi Yano and Asher Pasha and Yunchen Gong and Bi, {Yong Mei} and Nicolas Provart and David Guttman and Anne Krapp and Rothstein, {Steven J.} and Eiji Nambara",

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doi = "10.1038/ncomms13179",

language = "English",

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T1 - NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

AU - Yan, Dawei

AU - Easwaran, Vanathy

AU - Chau, Vivian

AU - Okamoto, Masanori

AU - Ierullo, Matthew

AU - Kimura, Mitsuhiro

AU - Endo, Akira

AU - Yano, Ryoichi

AU - Pasha, Asher

AU - Gong, Yunchen

AU - Bi, Yong Mei

AU - Provart, Nicolas

AU - Guttman, David

AU - Krapp, Anne

AU - Rothstein, Steven J.

AU - Nambara, Eiji

PY - 2016/10/12

Y1 - 2016/10/12

N2 - Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

AB - Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

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NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

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