Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis

Takahiro Joudoi, Yudai Shichiri, Nobuto Kamizono, Takaaki Akaike, Tomohiro Sawa, Jun Yoshitake, Naotaka Yamada, Sumio Iwai

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Nitric oxide (NO) is a ubiquitous signaling molecule involved in diverse physiological processes, including plant senescence and stomatal closure. The NO and cyclic GMP (cGMP) cascade is the main NO signaling pathway in animals, but whether this pathway operates in plant cells, and the mechanisms of its action, remain unclear. Here, we assessed the possibility that the nitrated cGMP derivative 8-nitro-cGMP functions in guard cell signaling. Mass spectrometry and immunocytochemical analyses showed that abscisic acid and NO induced the synthesis of 8-nitro-cGMP in guard cells in the presence of reactive oxygen species. 8-Nitro-cGMP triggered stomatal closure, but 8-bromoguanosine 39,59-cyclic monophosphate (8-bromocGMP), a membrane-permeating analog of cGMP, did not. However, in the dark, 8-bromo-cGMP induced stomatal opening but 8-nitro-cGMP did not. Thus, cGMP and its nitrated derivative play different roles in the signaling pathways that lead to stomatal opening and closure. Moreover, inhibitor and genetic studies showed that calcium, cyclic adenosine-59- diphosphate-ribose, and SLOW ANION CHANNEL1 act downstream of 8-nitro-cGMP. This study therefore demonstrates that 8-nitro-cGMP acts as a guard cell signaling molecule and that a NO/8-nitro-cGMP signaling cascade operates in guard cells.

Original languageEnglish
Pages (from-to)558-571
Number of pages14
JournalPlant Cell
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 1 2013

Fingerprint

cyclic GMP
Cyclic GMP
guard cells
Arabidopsis
Nitric Oxide
nitric oxide
Cyclic ADP-Ribose
Plant Physiological Phenomena
chemical derivatives
Abscisic Acid
Plant Cells
ribose
adenosine
Reactive Oxygen Species
Mass Spectrometry
abscisic acid
reactive oxygen species
mechanism of action

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Joudoi, T., Shichiri, Y., Kamizono, N., Akaike, T., Sawa, T., Yoshitake, J., ... Iwai, S. (2013). Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis. Plant Cell, 25(2), 558-571. https://doi.org/10.1105/tpc.112.105049

Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis. / Joudoi, Takahiro; Shichiri, Yudai; Kamizono, Nobuto; Akaike, Takaaki; Sawa, Tomohiro; Yoshitake, Jun; Yamada, Naotaka; Iwai, Sumio.

In: Plant Cell, Vol. 25, No. 2, 01.02.2013, p. 558-571.

Research output: Contribution to journalArticle

Joudoi, T, Shichiri, Y, Kamizono, N, Akaike, T, Sawa, T, Yoshitake, J, Yamada, N & Iwai, S 2013, 'Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis', Plant Cell, vol. 25, no. 2, pp. 558-571. https://doi.org/10.1105/tpc.112.105049
Joudoi T, Shichiri Y, Kamizono N, Akaike T, Sawa T, Yoshitake J et al. Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis. Plant Cell. 2013 Feb 1;25(2):558-571. https://doi.org/10.1105/tpc.112.105049
Joudoi, Takahiro ; Shichiri, Yudai ; Kamizono, Nobuto ; Akaike, Takaaki ; Sawa, Tomohiro ; Yoshitake, Jun ; Yamada, Naotaka ; Iwai, Sumio. / Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis. In: Plant Cell. 2013 ; Vol. 25, No. 2. pp. 558-571.
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