Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves

Misa Takahashi, Jun Shigeto, Shunsuke Izumi, Katsutoshi Yoshizato, Hiromichi Morikawa

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr36 of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.

Original languageEnglish
Article numbere1197464
JournalPlant Signaling and Behavior
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 2 2016

All Science Journal Classification (ASJC) codes

  • Plant Science

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