Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH)

Norjan Yusof, Ali Hassan Mohd Ali Hassan, Lai Yee Phang Lai Yee, Meisam Tabatabaei, Ridzuan Othman Mohd Ridzuan Othman, Masatsugu Mori, Minato Wakisaka, Kenji Sakai, Yoshihito Shirai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nitrification of mature sanitary landfill leachate with high-strength of N-NH4 + (1080-2350 mg L-1) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibition before being fed with actual mature leachate. Successful nitrification was achieved with an approximately complete ammonium removal (99%) and 96% of N-NH4 + conversion to N-NO- 3. The maximum volumetric and specific nitrification rates obtained were 2.56 kg N-NH4 + m-3 day-1 and 0.23 g N-NH4 + g-1 volatile suspended solid (VSS) day-1, respectively, at hydraulic retention time (HRT) of 12.7 h and solid retention time of 50 days. Incomplete nitrification was encountered when operating at a higher nitrogen loading rate of 3.14 kg N-NH4 + m-3 day-1. The substrate overloading and nitrifiers competition with heterotrophs were believed to trigger the incomplete nitrification. Fluorescence in situ hybridization (FISH) results supported the syntrophic association between the ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria. FISH results also revealed the heterotrophs as the dominant and disintegration of some AOB cell aggregates into single cells which further supported the incomplete nitrification phenomenon.

Original languageEnglish
Pages (from-to)602-611
Number of pages10
JournalWaste Management and Research
Volume29
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Nitrification
Land fill
Fluorescence
Bacteria
Ammonia
Disintegration
Substrates
Hydraulics
Association reactions
Nitrogen

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Pollution

Cite this

Yusof, N., Mohd Ali Hassan, A. H., Phang Lai Yee, L. Y., Tabatabaei, M., Mohd Ridzuan Othman, R. O., Mori, M., ... Shirai, Y. (2011). Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH). Waste Management and Research, 29(6), 602-611. https://doi.org/10.1177/0734242X10397581

Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH). / Yusof, Norjan; Mohd Ali Hassan, Ali Hassan; Phang Lai Yee, Lai Yee; Tabatabaei, Meisam; Mohd Ridzuan Othman, Ridzuan Othman; Mori, Masatsugu; Wakisaka, Minato; Sakai, Kenji; Shirai, Yoshihito.

In: Waste Management and Research, Vol. 29, No. 6, 01.06.2011, p. 602-611.

Research output: Contribution to journalArticle

Yusof, N, Mohd Ali Hassan, AH, Phang Lai Yee, LY, Tabatabaei, M, Mohd Ridzuan Othman, RO, Mori, M, Wakisaka, M, Sakai, K & Shirai, Y 2011, 'Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH)', Waste Management and Research, vol. 29, no. 6, pp. 602-611. https://doi.org/10.1177/0734242X10397581
Yusof, Norjan ; Mohd Ali Hassan, Ali Hassan ; Phang Lai Yee, Lai Yee ; Tabatabaei, Meisam ; Mohd Ridzuan Othman, Ridzuan Othman ; Mori, Masatsugu ; Wakisaka, Minato ; Sakai, Kenji ; Shirai, Yoshihito. / Nitrification of high-strength ammonium landfill leachate with microbial community analysis using fluorescence in situ hybridization (FISH). In: Waste Management and Research. 2011 ; Vol. 29, No. 6. pp. 602-611.
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