Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean

Md Matiul Islam, Yushi Ishibashi, Andressa C.S. Nakagawa, Yuki Tomita, Mari Iwaya-Inoue, Susumu Arima, Shao Hui Zheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

It is well known that some nitrogen in the vegetative organs is redistributed to the seeds during seed filling in soybean (Glycine max [L.] Merrill). This redistribution is considered to affect the seed yield of soybean. However, it is still not clear when the nitrogen moves from the vegetative part to the seeds, and the relationship between nitrogen redistribution and leaf senescence has not been clarified. The soybean variety Fukuyutaka was grown in the experimental field of Saga University, Japan from 22 July to 31 October, 2014. After the first flower stage (R1), the plant samples were collected weekly and were separated into leaf, petiole, stem, podshell and seed. The nitrogen concentrations in each plant part were determined. Fresh leaf samples were provided for the determination of soluble protein and autophagy gene GmATG8c expression. The nitrogen that accumulated in the vegetative parts reached its highest level at 60 days after sowing (DAS), then began to decrease at 73 DAS (R6). This decrease is considered to be the consequence of nitrogen redistribution from the vegetative parts to the seeds. The movement of nitrogen from the vegetative parts to the seeds was estimated to occur at around 73 DAS (R6). At this stage, leaf SPAD values, leaf nitrogen, and soluble protein concentrations began to decrease simultaneously, suggesting the onset of leaf senescence. Furthermore, the expression of the autophagy gene GmATG8c in the leaves increased dramatically from 73 to 85 DAS, which is the duration of nitrogen redistribution. The results suggest that the nitrogen redistribution from the vegetative parts to the seeds could be one of the initiating factors of leaf senescence, and the autophagy gene GmATG8c was associated with this process.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalJournal of Plant Physiology
Volume192
DOIs
Publication statusPublished - Mar 15 2016

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filling period
Soybeans
Seeds
Nitrogen
soybeans
nitrogen
autophagy
leaves
Autophagy
sowing
seeds
Gene Expression
plant anatomy
seed yield
Glycine max
Japan
Proteins
genes
proteins
flowers

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean. / Islam, Md Matiul; Ishibashi, Yushi; Nakagawa, Andressa C.S.; Tomita, Yuki; Iwaya-Inoue, Mari; Arima, Susumu; Zheng, Shao Hui.

In: Journal of Plant Physiology, Vol. 192, 15.03.2016, p. 71-74.

Research output: Contribution to journalArticle

Islam, Md Matiul ; Ishibashi, Yushi ; Nakagawa, Andressa C.S. ; Tomita, Yuki ; Iwaya-Inoue, Mari ; Arima, Susumu ; Zheng, Shao Hui. / Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean. In: Journal of Plant Physiology. 2016 ; Vol. 192. pp. 71-74.
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