Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry

Matthew R. Dreher, Martyna Elas, Kazuhiro Ichikawa, Eugene D. Barth, Ashutosh Chilkoti, Gerald M. Rosen, Howard J. Halpern, Mark Dewhirst

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    Hyperthermia, as an adjuvant with radiation and chemotherapy, has shown promise in the treatment of cancer. The relevant biological effects of a hyperthermia treatment are both time and temperature-dependent, creating a need for accurate thermometry. We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. When examined with electron paramagnetic resonance (EPR) spectroscopy, the ELP-nitroxide conjugate has temperature-dependent spectral line widths whose predictive accuracy is ∼0.3°C (80 μM). We believe that the temperature-dependent changes observed in the EPR spectrum are due to the combined effect of temperature, viscosity and effective radius on the rotational correlation time of the ELP-nitroxide conjugate.

    Original languageEnglish
    Pages (from-to)2755-2762
    Number of pages8
    JournalMedical physics
    Volume31
    Issue number10
    DOIs
    Publication statusPublished - Oct 2004

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Radiology Nuclear Medicine and imaging

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