NK T cell-derived IL-10 is essential for the differentiation of antigen-specific T regulatory cells in systemic tolerance

Kohei Sonoda, D. E. Faunce, M. Taniguchi, M. Exley, S. Balk, J. Stein-Streilein

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

In a model of systemic tolerance called Anterior Chamber-Associated Immune Deviation (ACAID), the differentiation of the T regulatory (Tr) cells depends on NK T cells and occurs in the spleen. We now show that the CD1d-reactive NK T cell subpopulation, required for development of systemic tolerance, expresses the invariant Vα14Jα281 TCR because Jα281 knockout (KO) mice were unable to generate Ag-specific Tr cells and ACAID. The mechanism for NK T cell-dependent differentiation of Ag-specific Tr cells mediating systemic tolerance was studied by defining the cytokine profiles in heterogeneous and enriched NK T spleen cells. In contrast to there being no differences in most regulatory cytokine mRNAs, both mRNA and protein for IL-10 were increased in splenic NK T cells of anterior chamber (a.c.)-inoculated mice. However, IL-10 mRNA was not increased in spleens after i.v. inoculation. Finally, NK T cells from wild-type (WT) mice, but not from IL-10 KO mice, reconstituted the ACAID inducing ability in Jα281 KO mice. Thus, NK T cell-derived IL-10 is critical for the generation of the Ag-specific Tr cells and systemic tolerance induced to eye-inoculated Ags.

Original languageEnglish
Pages (from-to)42-50
Number of pages9
JournalJournal of Immunology
Volume166
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Differentiation Antigens
Regulatory T-Lymphocytes
Natural Killer Cells
Interleukin-10
Anterior Chamber
T-Lymphocytes
Knockout Mice
Spleen
Messenger RNA
Cytokines
Cell Differentiation
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

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NK T cell-derived IL-10 is essential for the differentiation of antigen-specific T regulatory cells in systemic tolerance. / Sonoda, Kohei; Faunce, D. E.; Taniguchi, M.; Exley, M.; Balk, S.; Stein-Streilein, J.

In: Journal of Immunology, Vol. 166, No. 1, 01.01.2001, p. 42-50.

Research output: Contribution to journalArticle

Sonoda, Kohei ; Faunce, D. E. ; Taniguchi, M. ; Exley, M. ; Balk, S. ; Stein-Streilein, J. / NK T cell-derived IL-10 is essential for the differentiation of antigen-specific T regulatory cells in systemic tolerance. In: Journal of Immunology. 2001 ; Vol. 166, No. 1. pp. 42-50.
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