NMR solution structure of the tandem Src homology 3 domains of p47 phox complexed with a p22phox-derived proline-rich peptide

Kenji Ogura, Ikuo Nobuhisa, Satoru Yuzawa, Ryu Takeya, Shinnosuke Torikai, Kazuya Saikawa, Hideki Sumimoto, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The phagocyte NADPH oxidase plays a crucial role in host defense against microbial infections by generating reactive oxygen species. It is amultisubunit enzyme composed of membrane-bound flavocytochrome b558 as well as cytosolic components, including p47phox, which is essential for assembly of the complex. When phagocytes are activated, the cytosolic components of the NADPH oxidase translocate to flavocytochrome b558 due to binding of the tandem Src homology 3 (SH3) domains of p47phox to a proline-rich region in p22phox, a subunit of flavocytochrome b 558. Using NMR titration, we first identified the proline-rich region of p22phox that is essential for binding to the tandem SH3 domains of p47phox. We subsequently determined the solution structure of the p47phox tandem SH3 domains complexed with the proline-rich peptide of p22phox using NMR spectroscopy. In contrast to the intertwined dimer reported for the crystal state, the solution structure is amonomer. The central region of the p22phox peptide forms a polyproline type II helix that is sandwiched by the N- and C-terminal SH3 domains, as was observed in the crystal structure, whereas the C-terminal region of the peptide takes on a short α-helical conformation that provides an additional binding site with the N-terminal SH3 domain. Thus, the C-terminal α-helical region of the p22phox peptide increases the binding affinity for the tandem SH3 domains of p47phox more than 10-fold.

Original languageEnglish
Pages (from-to)3660-3668
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number6
DOIs
Publication statusPublished - Feb 10 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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