Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol

Yoko Ikunaga, Ikuo Sato, Stephanie Grond, Nobutaka Numaziri, Shigenobu Yoshida, Hiroko Yamaya, Syuntaro Hiradate, Morifumi Hasegawa, Hiroaki Toshima, Motoo Koitabashi, Michihiro Ito, Petr Karlovsky, Seiya Tsushima

Research output: Contribution to journalArticle

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Abstract

The mycotoxin deoxynivalenol (DON) causes serious problems worldwide in the production of crops such as wheat and barley because of its toxicity toward humans and livestock. A bacterial culture capable of degrading DON was obtained from soil samples collected in wheat fields using an enrichment culture procedure. The isolated bacterium, designated strain WSN05-2, completely removed 1,000 μg/mL of DON from the culture medium after incubation for 10 days. On the basis of phylogenetic studies, WSN05-2 was classified as a bacterium belonging to the genus Nocardioides. WSN05-2 showed significant growth in culture medium with DON as the sole carbon source. High-performance liquid chromatography analysis indicated the presence of a major initial metabolite of DON in the culture supernatant. The metabolite was identified as 3-epi-deoxynivalenol (3-epi-DON) by mass spectrometry and 1H and 13C nuclear magnetic resonance analysis. The amount of DON on wheat grain was reduced by about 90% at 7 days after inoculation with WSN05-2. This is the first report of a Nocardioides sp. strain able to degrade DON and of the yet unknown 3-epi-DON as an intermediate in the degradation of DON by a microorganism.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume89
Issue number2
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

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Triticum
Culture Media
deoxynivalenol
Bacteria
Mycotoxins
Livestock
Hordeum
Mass Spectrometry
Magnetic Resonance Spectroscopy
Soil
Carbon
High Pressure Liquid Chromatography
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. / Ikunaga, Yoko; Sato, Ikuo; Grond, Stephanie; Numaziri, Nobutaka; Yoshida, Shigenobu; Yamaya, Hiroko; Hiradate, Syuntaro; Hasegawa, Morifumi; Toshima, Hiroaki; Koitabashi, Motoo; Ito, Michihiro; Karlovsky, Petr; Tsushima, Seiya.

In: Applied Microbiology and Biotechnology, Vol. 89, No. 2, 01.01.2011, p. 419-427.

Research output: Contribution to journalArticle

Ikunaga, Y, Sato, I, Grond, S, Numaziri, N, Yoshida, S, Yamaya, H, Hiradate, S, Hasegawa, M, Toshima, H, Koitabashi, M, Ito, M, Karlovsky, P & Tsushima, S 2011, 'Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol', Applied Microbiology and Biotechnology, vol. 89, no. 2, pp. 419-427. https://doi.org/10.1007/s00253-010-2857-z
Ikunaga, Yoko ; Sato, Ikuo ; Grond, Stephanie ; Numaziri, Nobutaka ; Yoshida, Shigenobu ; Yamaya, Hiroko ; Hiradate, Syuntaro ; Hasegawa, Morifumi ; Toshima, Hiroaki ; Koitabashi, Motoo ; Ito, Michihiro ; Karlovsky, Petr ; Tsushima, Seiya. / Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. In: Applied Microbiology and Biotechnology. 2011 ; Vol. 89, No. 2. pp. 419-427.
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