Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol

Yoko Ikunaga, Ikuo Sato, Stephanie Grond, Nobutaka Numaziri, Shigenobu Yoshida, Hiroko Yamaya, Syuntaro Hiradate, Morifumi Hasegawa, Hiroaki Toshima, Motoo Koitabashi, Michihiro Ito, Petr Karlovsky, Seiya Tsushima

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

The mycotoxin deoxynivalenol (DON) causes serious problems worldwide in the production of crops such as wheat and barley because of its toxicity toward humans and livestock. A bacterial culture capable of degrading DON was obtained from soil samples collected in wheat fields using an enrichment culture procedure. The isolated bacterium, designated strain WSN05-2, completely removed 1,000 μg/mL of DON from the culture medium after incubation for 10 days. On the basis of phylogenetic studies, WSN05-2 was classified as a bacterium belonging to the genus Nocardioides. WSN05-2 showed significant growth in culture medium with DON as the sole carbon source. High-performance liquid chromatography analysis indicated the presence of a major initial metabolite of DON in the culture supernatant. The metabolite was identified as 3-epi-deoxynivalenol (3-epi-DON) by mass spectrometry and 1H and 13C nuclear magnetic resonance analysis. The amount of DON on wheat grain was reduced by about 90% at 7 days after inoculation with WSN05-2. This is the first report of a Nocardioides sp. strain able to degrade DON and of the yet unknown 3-epi-DON as an intermediate in the degradation of DON by a microorganism.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume89
Issue number2
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol'. Together they form a unique fingerprint.

Cite this