Non-coding RNA directed DNA demethylation of Sphk1 CpG island

Takuya Imamura, Soshi Yamamoto, Jun Ohgane, Naka Hattori, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The formation of DNA methylation patterns is one of the epigenetic events that underlie mammalian development. The Sphk1 CpG island is a target for tissue-dependent DNA methylation as well as a template for generating multiple subtypes. The number of mammalian non-coding RNA genes is rapidly expanding. In this study, we found endogenous antisense transcripts, Khps1 subtypes with different sizes (600-20,000 nt). A subtype, Khps1a, was a 1290-bp, non-coding, 5′-capped and 3′-polyadenylated RNA that originated from the CpG island and overlapped with a tissue-dependent differentially methylated region (T-DMR) of Sphk1. Intriguingly, overexpression of two fragments of Khps1 caused demethylation of CG sites in the T-DMR. Furthermore, this RNA-directed demethylation was associated with DNA methylation at three CC(A/T)GG sites in the T-DMR. The link between the RNA-directed CG demethylation and non-CG methylation provides a novel mechanism of epigenetic regulation and potential tool for epigenetic manipulation of mammalian cells.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume322
Issue number2
DOIs
Publication statusPublished - Sep 17 2004
Externally publishedYes

Fingerprint

Untranslated RNA
CpG Islands
DNA Methylation
Epigenomics
Tissue
DNA
RNA
Methylation
Genes
Cells
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Non-coding RNA directed DNA demethylation of Sphk1 CpG island. / Imamura, Takuya; Yamamoto, Soshi; Ohgane, Jun; Hattori, Naka; Tanaka, Satoshi; Shiota, Kunio.

In: Biochemical and Biophysical Research Communications, Vol. 322, No. 2, 17.09.2004, p. 593-600.

Research output: Contribution to journalArticle

Imamura, Takuya ; Yamamoto, Soshi ; Ohgane, Jun ; Hattori, Naka ; Tanaka, Satoshi ; Shiota, Kunio. / Non-coding RNA directed DNA demethylation of Sphk1 CpG island. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 322, No. 2. pp. 593-600.
@article{f8e2223022fe40ec9e0b990fd21f88e2,
title = "Non-coding RNA directed DNA demethylation of Sphk1 CpG island",
abstract = "The formation of DNA methylation patterns is one of the epigenetic events that underlie mammalian development. The Sphk1 CpG island is a target for tissue-dependent DNA methylation as well as a template for generating multiple subtypes. The number of mammalian non-coding RNA genes is rapidly expanding. In this study, we found endogenous antisense transcripts, Khps1 subtypes with different sizes (600-20,000 nt). A subtype, Khps1a, was a 1290-bp, non-coding, 5′-capped and 3′-polyadenylated RNA that originated from the CpG island and overlapped with a tissue-dependent differentially methylated region (T-DMR) of Sphk1. Intriguingly, overexpression of two fragments of Khps1 caused demethylation of CG sites in the T-DMR. Furthermore, this RNA-directed demethylation was associated with DNA methylation at three CC(A/T)GG sites in the T-DMR. The link between the RNA-directed CG demethylation and non-CG methylation provides a novel mechanism of epigenetic regulation and potential tool for epigenetic manipulation of mammalian cells.",
author = "Takuya Imamura and Soshi Yamamoto and Jun Ohgane and Naka Hattori and Satoshi Tanaka and Kunio Shiota",
year = "2004",
month = "9",
day = "17",
doi = "10.1016/j.bbrc.2004.07.159",
language = "English",
volume = "322",
pages = "593--600",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Non-coding RNA directed DNA demethylation of Sphk1 CpG island

AU - Imamura, Takuya

AU - Yamamoto, Soshi

AU - Ohgane, Jun

AU - Hattori, Naka

AU - Tanaka, Satoshi

AU - Shiota, Kunio

PY - 2004/9/17

Y1 - 2004/9/17

N2 - The formation of DNA methylation patterns is one of the epigenetic events that underlie mammalian development. The Sphk1 CpG island is a target for tissue-dependent DNA methylation as well as a template for generating multiple subtypes. The number of mammalian non-coding RNA genes is rapidly expanding. In this study, we found endogenous antisense transcripts, Khps1 subtypes with different sizes (600-20,000 nt). A subtype, Khps1a, was a 1290-bp, non-coding, 5′-capped and 3′-polyadenylated RNA that originated from the CpG island and overlapped with a tissue-dependent differentially methylated region (T-DMR) of Sphk1. Intriguingly, overexpression of two fragments of Khps1 caused demethylation of CG sites in the T-DMR. Furthermore, this RNA-directed demethylation was associated with DNA methylation at three CC(A/T)GG sites in the T-DMR. The link between the RNA-directed CG demethylation and non-CG methylation provides a novel mechanism of epigenetic regulation and potential tool for epigenetic manipulation of mammalian cells.

AB - The formation of DNA methylation patterns is one of the epigenetic events that underlie mammalian development. The Sphk1 CpG island is a target for tissue-dependent DNA methylation as well as a template for generating multiple subtypes. The number of mammalian non-coding RNA genes is rapidly expanding. In this study, we found endogenous antisense transcripts, Khps1 subtypes with different sizes (600-20,000 nt). A subtype, Khps1a, was a 1290-bp, non-coding, 5′-capped and 3′-polyadenylated RNA that originated from the CpG island and overlapped with a tissue-dependent differentially methylated region (T-DMR) of Sphk1. Intriguingly, overexpression of two fragments of Khps1 caused demethylation of CG sites in the T-DMR. Furthermore, this RNA-directed demethylation was associated with DNA methylation at three CC(A/T)GG sites in the T-DMR. The link between the RNA-directed CG demethylation and non-CG methylation provides a novel mechanism of epigenetic regulation and potential tool for epigenetic manipulation of mammalian cells.

UR - http://www.scopus.com/inward/record.url?scp=6044266260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6044266260&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.07.159

DO - 10.1016/j.bbrc.2004.07.159

M3 - Article

C2 - 15325271

AN - SCOPUS:6044266260

VL - 322

SP - 593

EP - 600

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -