Non-crosslinking aggregation of DNA-functionalized gold nanoparticles for gene diagnosis and directed assembly

Guoqing Wang, Yoshitsugu Akiyama, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A gold nanoparticle densely modified with DNA (DNA 'AuNP), which integrates the ability of molecular recognition with plasmonic properties, is a well-established platform in nanobiotechnology. Both in analytical science and materials science, DNA 'AuNP assemblies crosslinked by Watson-Crick base pairing have gained great popularity. Yet the finding of non-crosslinking aggregation of DNA 'AuNPs has brought us to another fascinating side of this material. Upon hybridization with the complementary DNA, the DNA 'AuNPs undergo a dramatic decrease in colloidal stability accompanied with a distinct color change due to blunt-end stacking interactions. When hybridized to the terminal-mismatched DNA, the DNA 'AuNPs stay dispersed even at high ionic strength by virtue of fraying motion of the outmost unpaired nucleobases. This distinct colloidal phenomenon provides a colorimetric approach to discriminate single-nucleotide polymorphisms. Furthermore, by region-specific arrangement of single-base pairing/unpairing at the outmost surface of the DNA layer, gold nanorods can spontaneously evolve to assemble in an orientation-controlled fashion, promising applications in fabrication of various nanodevices.

Original languageEnglish
Title of host publicationTargeted Nanosystems for Therapeutic Applications
Subtitle of host publicationNew Concepts, Dynamic Properties, Efficiency, and Toxicity
EditorsKazuo Sakurai, Marc A. Ilies
PublisherAmerican Chemical Society
Pages119-138
Number of pages20
ISBN (Electronic)9780841233836
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Publication series

NameACS Symposium Series
Volume1309
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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  • Cite this

    Wang, G., Akiyama, Y., Kanayama, N., Takarada, T., & Maeda, M. (2019). Non-crosslinking aggregation of DNA-functionalized gold nanoparticles for gene diagnosis and directed assembly. In K. Sakurai, & M. A. Ilies (Eds.), Targeted Nanosystems for Therapeutic Applications: New Concepts, Dynamic Properties, Efficiency, and Toxicity (pp. 119-138). (ACS Symposium Series; Vol. 1309). American Chemical Society. https://doi.org/10.1021/bk-2019-1309.ch006