Non-enzymatic covalent protein labeling using a reactive tag

Hiroshi Nonaka, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.

Original languageEnglish
Pages (from-to)15777-15779
Number of pages3
JournalJournal of the American Chemical Society
Volume129
Issue number51
DOIs
Publication statusPublished - Dec 26 2007
Externally publishedYes

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Labeling
Proteins
Molecular Probes
Molecular recognition
Peptides
Experiments

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Non-enzymatic covalent protein labeling using a reactive tag. / Nonaka, Hiroshi; Tsukiji, Shinya; Ojida, Akio; Hamachi, Itaru.

In: Journal of the American Chemical Society, Vol. 129, No. 51, 26.12.2007, p. 15777-15779.

Research output: Contribution to journalArticle

Nonaka, Hiroshi ; Tsukiji, Shinya ; Ojida, Akio ; Hamachi, Itaru. / Non-enzymatic covalent protein labeling using a reactive tag. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 51. pp. 15777-15779.
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