Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells

Takafumi Hiramoto, Maino Tahara, Jiyuan Liao, Yasushi Soda, Yoshie Miura, Ryo Kurita, Hiroshi Hamana, Kota Inoue, Hiroshi Kohara, Shohei Miyamoto, Yasuki Hijikata, Shinji Okano, Yoshiyuki Yamaguchi, Yoshinao Oda, Kenji Ichiyanagi, Hidehiro Toh, Hiroyuki Sasaki, Hiroyuki Kishi, Akihide Ryo, Atsushi MuraguchiMakoto Takeda, Kenzaburo Tani

Research output: Contribution to journalArticle

Abstract

Recent advances in gene therapy technologies have enabled the treatment of congenital disorders and cancers and facilitated the development of innovative methods, including induced pluripotent stem cell (iPSC) production and genome editing. We recently developed a novel non-transmissible and non-integrating measles virus (MV) vector capable of transferring multiple genes simultaneously into a wide range of cells through the CD46 and CD150 receptors. The MV vector expresses four genes for iPSC generation and the GFP gene for a period of time sufficient to establish iPSCs from human fibroblasts as well as peripheral blood T cells. The transgenes were expressed differentially depending on their gene order in the vector. Human hematopoietic stem/progenitor cells were directly and efficiently reprogrammed to naive-like cells that could proliferate and differentiate into primed iPSCs by the same method used to establish primed iPSCs from other cell types. The novel MV vector has several advantages for establishing iPSCs and potential future applications in gene therapy. This new non-transmissible and non-integrating measles virus vector, which can transfer multiple genes simultaneously into a wide range of cells through the CD46 and CD150 receptors and induce primed or naive-like pluripotent stem cells from hematopoietic cells in the same condition, will definitely contribute to the gene and cell therapy.

Original languageEnglish
JournalMolecular Therapy
DOIs
Publication statusAccepted/In press - 2019

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

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    Hiramoto, T., Tahara, M., Liao, J., Soda, Y., Miura, Y., Kurita, R., Hamana, H., Inoue, K., Kohara, H., Miyamoto, S., Hijikata, Y., Okano, S., Yamaguchi, Y., Oda, Y., Ichiyanagi, K., Toh, H., Sasaki, H., Kishi, H., Ryo, A., ... Tani, K. (Accepted/In press). Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells. Molecular Therapy. https://doi.org/10.1016/j.ymthe.2019.09.007