Noninvasive in vivo oximetric imaging by radiofrequency FT EPR

Sankaran Subramanian, Ken-Ichi Yamada, Akira Irie, Ramachandran Murugesan, John A. Cook, Nallathamby Devasahayam, Gootzian M. Van Dam, James B. Mitchell, Murali C. Krishna

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A novel method, called relaxo-oximetry, for rapid spatially resolved in vivo measurements of oxygen concentration using time-domain radiofrequency (RF) electron paramagnetic resonance (EPR) is described. Time-domain data from triaryl methyl (TAM)-based single-electron contrast agents were processed by systematic deletion of the initial time points to arrive at T2*-weighted discrimination of signal amplitudes. In experiments involving phantoms, the line widths [∼ (T2*)-1] increased as a function of oxygen, and the slope (line width/pO2) was the same for both absorption- and magnitude-mode line shapes. Line widths derived from T2* weighting and the computed pO2 values agreed favorably with the measured ones from phantoms of known oxygen tension. In vivo relaxo-oximetry was performed on C3H mice, and it was found that the liver was more hypoxic than the kidneys. For tumors, 2D oxygen maps were generated while the animal breathed room air or Carbogen® (95% O2/5% CO2). Carbogen® enhanced oxygen concentration within the tumor, and the pO2 histograms showed considerable heterogeneity. Clark electrode oxygen measurements on organs and tumors were in good agreement with tissue oxygen measurements done by relaxo-oximetry. Thus, from a single spatial image data set, pO2 measurements can be done noninvasively by relaxo-oximetry, and 3D imaging can be performed in less than 3 min.

Original languageEnglish
Pages (from-to)1001-1008
Number of pages8
JournalMagnetic Resonance in Medicine
Volume47
Issue number5
DOIs
Publication statusPublished - May 9 2002
Externally publishedYes

Fingerprint

Electron Spin Resonance Spectroscopy
Oxygen
Oximetry
Neoplasms
Inbred C3H Mouse
Contrast Media
Electrodes
Air
Electrons
Kidney
Liver

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Subramanian, S., Yamada, K-I., Irie, A., Murugesan, R., Cook, J. A., Devasahayam, N., ... Krishna, M. C. (2002). Noninvasive in vivo oximetric imaging by radiofrequency FT EPR. Magnetic Resonance in Medicine, 47(5), 1001-1008. https://doi.org/10.1002/mrm.10133

Noninvasive in vivo oximetric imaging by radiofrequency FT EPR. / Subramanian, Sankaran; Yamada, Ken-Ichi; Irie, Akira; Murugesan, Ramachandran; Cook, John A.; Devasahayam, Nallathamby; Van Dam, Gootzian M.; Mitchell, James B.; Krishna, Murali C.

In: Magnetic Resonance in Medicine, Vol. 47, No. 5, 09.05.2002, p. 1001-1008.

Research output: Contribution to journalArticle

Subramanian, S, Yamada, K-I, Irie, A, Murugesan, R, Cook, JA, Devasahayam, N, Van Dam, GM, Mitchell, JB & Krishna, MC 2002, 'Noninvasive in vivo oximetric imaging by radiofrequency FT EPR', Magnetic Resonance in Medicine, vol. 47, no. 5, pp. 1001-1008. https://doi.org/10.1002/mrm.10133
Subramanian S, Yamada K-I, Irie A, Murugesan R, Cook JA, Devasahayam N et al. Noninvasive in vivo oximetric imaging by radiofrequency FT EPR. Magnetic Resonance in Medicine. 2002 May 9;47(5):1001-1008. https://doi.org/10.1002/mrm.10133
Subramanian, Sankaran ; Yamada, Ken-Ichi ; Irie, Akira ; Murugesan, Ramachandran ; Cook, John A. ; Devasahayam, Nallathamby ; Van Dam, Gootzian M. ; Mitchell, James B. ; Krishna, Murali C. / Noninvasive in vivo oximetric imaging by radiofrequency FT EPR. In: Magnetic Resonance in Medicine. 2002 ; Vol. 47, No. 5. pp. 1001-1008.
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