Noninvasive sensing of skin cancer for early diagnosis using cathespin L

Masaki Yamaguchi, Akira Date, Wataru Suzuki, Yusuke Tahara, Makoto Sasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is reported that Cathepsin L (CatL) activity is correlated with actinic keratosis and squamous cell cancers. The purpose of this study is to propose an on-site testing of CatL activity in the skin, which can be used for the diagnosis of skin conditions noninvasively, easily, quickly and at any time. In this study, a novel immunochromatographic test-strip is proposed for CatL analysis in the skin. In order to realize the disposable test-strip, a colloidal gold technique was selected as the molecular recognition method for the CatL. A human skin sample was collected using tape stripping. The fabricated immuno-chromatographic test-strip (test-strip) consists of four pads, a sample pad, a conjugate release pad, a nitrocellulose membrane and an absorption pad. Based on optimal assay conditions, the sensitivity of the test-strip was measured. It required 90 s for a sample to reach the test-line on the test-strip. The optical density was proportion to the activity of CatL. In order to cancel out the influence of the collecting technique, the CatL/TP ratio was evaluated. It was suggested that the test-strip might be used for the qualitative analysis of CatL in the stratum corneum.

Original languageEnglish
Title of host publicationProceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
Pages43-47
Number of pages5
Publication statusPublished - Dec 1 2010
Externally publishedYes
Event7th IASTED International Conference on Biomedical Engineering, BioMED 2010 - Innsbruck, Austria
Duration: Feb 17 2010Feb 19 2010

Publication series

NameProceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010
Volume2

Other

Other7th IASTED International Conference on Biomedical Engineering, BioMED 2010
CountryAustria
CityInnsbruck
Period2/17/102/19/10

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All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine (miscellaneous)

Cite this

Yamaguchi, M., Date, A., Suzuki, W., Tahara, Y., & Sasaki, M. (2010). Noninvasive sensing of skin cancer for early diagnosis using cathespin L. In Proceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010 (pp. 43-47). (Proceedings of the 7th IASTED International Conference on Biomedical Engineering, BioMED 2010; Vol. 2).