NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47

Takeshi Sekiguchi, Toshiya Hayano, Mitsuaki Yanagida, Nobuhiro Takahashi, Takeharu Nishimoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previously, we described a novel nucleolar protein, NOP132, which interacts with the small GTP binding protein RRAG A. To elucidate the function of NOP132 in the nucleolus, we identified proteins that interact with NOP132 using mass spectrometric methods. NOP132 associated mainly with proteins involved in ribosome biogenesis and RNA metabolism, including the DEAD-box RNA helicase protein, DDX47, whose yeast homolog is Rrp3, which has roles in pre-rRNA processing. Immunoprecipitation of FLAG-tagged DDX47 co-precipitated rRNA precursors, as well as a number of proteins that are probably involved in ribosome biogenesis, implying that DDX47 plays a role in pre-rRNA processing. Introduction of NOP132 small interfering RNAs induced a ring-like localization of DDX47 in the nucleolus, suggesting that NOP132 is required for the appropriate localization of DDX47 within the nucleolus. We propose that NOP132 functions in the recruitment of pre-rRNA processing proteins, including DDX47, to the region where rRNA is transcribed within the nucleolus.

Original languageEnglish
Pages (from-to)4593-4608
Number of pages16
JournalNucleic acids research
Volume34
Issue number16
DOIs
Publication statusPublished - Sep 1 2006

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DEAD-box RNA Helicases
RNA Precursors
Proteins
Ribosomes
Nuclear Proteins
GTP-Binding Proteins
Immunoprecipitation
Small Interfering RNA
Yeasts
RNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. / Sekiguchi, Takeshi; Hayano, Toshiya; Yanagida, Mitsuaki; Takahashi, Nobuhiro; Nishimoto, Takeharu.

In: Nucleic acids research, Vol. 34, No. 16, 01.09.2006, p. 4593-4608.

Research output: Contribution to journalArticle

Sekiguchi, Takeshi ; Hayano, Toshiya ; Yanagida, Mitsuaki ; Takahashi, Nobuhiro ; Nishimoto, Takeharu. / NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. In: Nucleic acids research. 2006 ; Vol. 34, No. 16. pp. 4593-4608.
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