Norepinephrine does not alter NPY and POMC mRNA expression in neonatal chicks

Sachiko Katayama, Shozo Tomonaga, Momoka Sato, Haruka Yamane, Yousuke Tsuneyoshi, D. Michael Denbow, Mitsuhiro Furuse

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Norepinephrine (NE), synthesized in both the central and peripheral nervous system, is involved in food intake regulation of both mammals and chickens. Neuropeptide Y (NPY), a potent orexigenic peptide, is colocalized with NE neurons in the central and peripheral nervous system, suggesting an interaction. Proopiomelanocortin (POMC) is the precursor of α-melanocyte stimulating hormone, a potent anorexigenic peptide synthesized in the hypothalamus. In this study, two experiments were conducted to examine the effect of intracerebroventricular (ICV) injection of NE on appetite mediators in neonatal chicks (Gallus gallus). Experiment 1 was done to confirm the effect of centrally administered NE (0, 25, 50, and 100 μg) on food intake following a 3 h fast, and to determine the change in NPY mRNA expression in the central nervous system (CNS). In Experiment 2, chicks fed ad libitum were treated ICV with NE (50 μg) to determine if changes occurred in brain NPY and POMC mRNA levels. In Experiment 1, the ICV injection of NE dose-dependently reduced food intake, but there was no change in NPY mRNA expression in the CNS. In Experiment 2, there was no significant change in NPY and POMC mRNA expression between the control and NE-treated group, indicating that ICV injection of NE may not be associated with changes in NPY or POMC gene expression.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume156
Issue number1
DOIs
Publication statusPublished - May 1 2010

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Pro-Opiomelanocortin
Neuropeptide Y
Norepinephrine
Messenger RNA
Neurology
Central Nervous System
Peripheral Nervous System
Experiments
Injections
Chickens
Eating
Melanocyte-Stimulating Hormones
Appetite Regulation
Mammals
Appetite
Gene expression
Hypothalamus
Neurons
Brain
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Molecular Biology

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Norepinephrine does not alter NPY and POMC mRNA expression in neonatal chicks. / Katayama, Sachiko; Tomonaga, Shozo; Sato, Momoka; Yamane, Haruka; Tsuneyoshi, Yousuke; Denbow, D. Michael; Furuse, Mitsuhiro.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 156, No. 1, 01.05.2010, p. 143-146.

Research output: Contribution to journalArticle

Katayama, Sachiko ; Tomonaga, Shozo ; Sato, Momoka ; Yamane, Haruka ; Tsuneyoshi, Yousuke ; Denbow, D. Michael ; Furuse, Mitsuhiro. / Norepinephrine does not alter NPY and POMC mRNA expression in neonatal chicks. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2010 ; Vol. 156, No. 1. pp. 143-146.
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