Normal lysosomal morphology and function in LAMP-1-deficient mice

Nicole Andrejewski, Eeva Liisa Punnonen, Gundula Guhde, Yoshitaka Tanaka, Renate Lüllmann-Rauch, Dieter Hartmann, Kurt Von Figura, Paul Saftig

Research output: Contribution to journalArticle

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Abstract

Lysosomal membranes contain two highly glycosylated proteins, designated LAMP-1 anti LAMP-2, as major components. LAMP-1 and LAMP-2 are structurally related. To investigate the physiological role of LAMP-1, we have generated mice deficient for this protein. LAMP-1-deficient mice are viable and fertile. In LAMP-1-deficient brain, a mild regional astrogliosis and altered immunoreactivity against cathepsin-D was observed. Histological and ultrastructural analyses of all other tissues did not reveal abnormalities. Lysosomal properties, such as enzyme activities, lysosomal pH, osmotic stability, density, shape, and subcellular distribution were not changed in comparison with controls. Western blot analyses of LAMP-1-deficient and heterozygote tissues revealed an up-regulation of the LAMP-2 protein pointing to a compensatory effect of LAMP-2 in response to the LAMP-1 deficiency. The increase of LAMP-2 was neither correlated with an increase in the level of lamp-2 mRNAs nor with increased half-life time of LAMP-2. This findings suggest a translational regulation of LAMP-2 expression.

Original languageEnglish
Pages (from-to)12692-12701
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number18
DOIs
Publication statusPublished - Apr 30 1999
Externally publishedYes

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Lysosome-Associated Membrane Glycoproteins
Tissue
Cathepsin D
Enzyme activity
Heterozygote
Electric lamps
Half-Life
Brain
Proteins
Up-Regulation
Western Blotting
Membranes
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Andrejewski, N., Punnonen, E. L., Guhde, G., Tanaka, Y., Lüllmann-Rauch, R., Hartmann, D., ... Saftig, P. (1999). Normal lysosomal morphology and function in LAMP-1-deficient mice. Journal of Biological Chemistry, 274(18), 12692-12701. https://doi.org/10.1074/jbc.274.18.12692

Normal lysosomal morphology and function in LAMP-1-deficient mice. / Andrejewski, Nicole; Punnonen, Eeva Liisa; Guhde, Gundula; Tanaka, Yoshitaka; Lüllmann-Rauch, Renate; Hartmann, Dieter; Von Figura, Kurt; Saftig, Paul.

In: Journal of Biological Chemistry, Vol. 274, No. 18, 30.04.1999, p. 12692-12701.

Research output: Contribution to journalArticle

Andrejewski, N, Punnonen, EL, Guhde, G, Tanaka, Y, Lüllmann-Rauch, R, Hartmann, D, Von Figura, K & Saftig, P 1999, 'Normal lysosomal morphology and function in LAMP-1-deficient mice', Journal of Biological Chemistry, vol. 274, no. 18, pp. 12692-12701. https://doi.org/10.1074/jbc.274.18.12692
Andrejewski N, Punnonen EL, Guhde G, Tanaka Y, Lüllmann-Rauch R, Hartmann D et al. Normal lysosomal morphology and function in LAMP-1-deficient mice. Journal of Biological Chemistry. 1999 Apr 30;274(18):12692-12701. https://doi.org/10.1074/jbc.274.18.12692
Andrejewski, Nicole ; Punnonen, Eeva Liisa ; Guhde, Gundula ; Tanaka, Yoshitaka ; Lüllmann-Rauch, Renate ; Hartmann, Dieter ; Von Figura, Kurt ; Saftig, Paul. / Normal lysosomal morphology and function in LAMP-1-deficient mice. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 18. pp. 12692-12701.
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