Notch Mediates the Segmental Specification of Angioblasts in Somites and Their Directed Migration toward the Dorsal Aorta in Avian Embryos

Yuki Sato, Tadayoshi Watanabe, Daisuke Saito, Teruaki Takahashi, Shosei Yoshida, Jun Kohyama, Emi Ohata, Hideyuki Okano, Yoshiko Takahashi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We studied, using avian embryos, mechanisms underlying the three-dimensional assembly of the dorsal aorta, the first-forming embryonic vessel in amniotes. This vessel originates from two distinct cell populations, the splanchnic and somitic mesoderms. We have unveiled a role for Notch signaling in the somitic contribution. Upon activation of Notch signaling, a subpopulation of cells in the posterior half of individual somites migrates ventrally toward the primary dorsal aorta of splanchnic origin. After reaching the primary aorta, these somitic cells differentiate into the definitive aortic endothelial cells. This Notch-induced ventral migration is mediated by EphrinB2 and by an attractant action of the primary aorta. Furthermore, long-term chasing of cells by transposon-mediated gene transfer reveals that the segmentally provided endothelial cells of somitic origin in the dorsal aorta ultimately populate the entire region of the vessel. We demonstrate the molecular and cellular mechanisms underlying the formation of embryonic blood vessels from mesenchymal cells.

Original languageEnglish
Pages (from-to)890-901
Number of pages12
JournalDevelopmental Cell
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 10 2008
Externally publishedYes

Fingerprint

Somites
Endothelial cells
Aorta
Embryonic Structures
Cells
Gene transfer
Specifications
Blood vessels
Viscera
Chemical activation
Endothelial Cells
Mesoderm
Blood Vessels
Population
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Notch Mediates the Segmental Specification of Angioblasts in Somites and Their Directed Migration toward the Dorsal Aorta in Avian Embryos. / Sato, Yuki; Watanabe, Tadayoshi; Saito, Daisuke; Takahashi, Teruaki; Yoshida, Shosei; Kohyama, Jun; Ohata, Emi; Okano, Hideyuki; Takahashi, Yoshiko.

In: Developmental Cell, Vol. 14, No. 6, 10.06.2008, p. 890-901.

Research output: Contribution to journalArticle

Sato, Yuki ; Watanabe, Tadayoshi ; Saito, Daisuke ; Takahashi, Teruaki ; Yoshida, Shosei ; Kohyama, Jun ; Ohata, Emi ; Okano, Hideyuki ; Takahashi, Yoshiko. / Notch Mediates the Segmental Specification of Angioblasts in Somites and Their Directed Migration toward the Dorsal Aorta in Avian Embryos. In: Developmental Cell. 2008 ; Vol. 14, No. 6. pp. 890-901.
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