Novel 3β-hydroxysteroid dehydrogenases from gonads of the Nile tilapia: Phylogenetic significance and expression during reproductive cycle

B. Senthilkumaran, C. C. Sudhakumari, D. S. Wang, G. Sreenivasulu, T. Kobayashi, H. K. Kobayashi, Michiyasu Yoshikuni, Y. Nagahama

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18 Citations (Scopus)

Abstract

Multiple forms of 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD) and their differential tissue expression pattern have not been shown in any lower vertebrates. In the present study, we report cloning of two novel 3β-HSDs and two variants from gonads of the Nile tilapia. 3β-HSD cDNAs encode two peptides of 375 (3β-HSD type-I/variant 1) and 367 (3β-HSD type-II/variant 1) amino acid residues that share 31.9% homology. 3β-HSD type-I/variant 1 shared high homology with other piscine counterparts while 3β-HSD type-II/variant 1 exhibited homology to mammalian ΔC27-3β-HSD and multifunctional viral 3β-HSD. The latter seems to be ancient form among vertebrates. Transiently transfected 3β-HSDs' open reading frames in COS-7 cells converted exogenous pregnenolone/androsta-5-ene-3β-17β-diol to progesterone/testosterone. Tissue distribution pattern of 3β-HSDs by RT-PCR revealed varied expression pattern. Northern blot analysis of 3β-HSDs demonstrated steady or gradual rise in transcripts level at different gonadal stages. These data revealed the importance of novel 3β-HSDs in teleosts and also provided phylogenetic significance.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume299
Issue number2
DOIs
Publication statusPublished - Feb 27 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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    Senthilkumaran, B., Sudhakumari, C. C., Wang, D. S., Sreenivasulu, G., Kobayashi, T., Kobayashi, H. K., Yoshikuni, M., & Nagahama, Y. (2009). Novel 3β-hydroxysteroid dehydrogenases from gonads of the Nile tilapia: Phylogenetic significance and expression during reproductive cycle. Molecular and Cellular Endocrinology, 299(2), 146-152. https://doi.org/10.1016/j.mce.2008.11.008