Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

Naohiko Shimada, Kentaro Muraki, Taro Anai, Arihiro Kano, Atsushi Maruyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

Original languageEnglish
Pages (from-to)339-340
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - Jan 1 2007

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Comb and Wattles
Single Nucleotide Polymorphism
Betaine
Single-Stranded DNA
DNA
DNA Probes
Alleles

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers. / Shimada, Naohiko; Muraki, Kentaro; Anai, Taro; Kano, Arihiro; Maruyama, Atsushi.

In: Nucleic acids symposium series (2004), No. 51, 01.01.2007, p. 339-340.

Research output: Contribution to journalArticle

Shimada, Naohiko ; Muraki, Kentaro ; Anai, Taro ; Kano, Arihiro ; Maruyama, Atsushi. / Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 339-340.
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