Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

Naohiko Shimada, Kentaro Muraki, Taro Anai, Arihiro Kano, Atsushi Maruyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

Original languageEnglish
Pages (from-to)339-340
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
DOIs
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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