Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

Naohiko Shimada; Kentaro Muraki; Taro Anai; Arihiro Kano; Atsushi Maruyama

doi:10.1093/nass/nrm170

Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

Naohiko Shimada, Kentaro Muraki, Taro Anai, Arihiro Kano, Atsushi Maruyama

Department of Fundamental Organic Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

Original language	English
Pages (from-to)	339-340
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
DOIs	https://doi.org/10.1093/nass/nrm170
Publication status	Published - 2007

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1093/nass/nrm170

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title = "Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.",

abstract = "We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.",

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year = "2007",

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T1 - Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

AU - Shimada, Naohiko

AU - Muraki, Kentaro

AU - Anai, Taro

AU - Kano, Arihiro

AU - Maruyama, Atsushi

PY - 2007

Y1 - 2007

N2 - We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

AB - We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.

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JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

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ER -

Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.

Abstract

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