Novel biological functions of interleukin-4: Formation of tube-like structures by vascular endothelial cells in vitro and angiogenesis in vivo

Jun-Ichi Fukushi, Takashi Morisaki, Tadahisa Shono, Akihiro Nishie, Hitoe Torisu, Mayumi Ono, Michihiko Kuwano

Research output: Contribution to journalArticle

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Abstract

The effect of interleukin-4 (IL-4) on angiogenesis was studied in vitro and in vivo. Human recombinant IL-4 significantly stimulated the formation of tube-like structures in collagen gels by bovine aortic endothelial cells as well as by human microvascular endothelial cells. Human recombinant IL-4 at 50-500 U/ml stimulated by about two- to threefold the formation of tubes by bovine aortic endothelial cells; the rate was comparable to that of basic fibroblast growth factor. Tube formation was almost completely inhibited by the addition of IL-4 receptor neutralizing antibody. Administration of rat recombinant IL-4 led to neovascularization when implanted in the rat cornea. Findings suggest that IL-4 may be a mediator of the immune system as well as an inducer of angiogenesis.

Original languageEnglish
Pages (from-to)444-448
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume250
Issue number2
DOIs
Publication statusPublished - Sep 18 1998

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Endothelial cells
Interleukin-4
Endothelial Cells
Rats
Interleukin-4 Receptors
Angiogenesis Inducing Agents
Immune system
Fibroblast Growth Factor 2
Neutralizing Antibodies
Cornea
Immune System
Collagen
Gels
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Novel biological functions of interleukin-4 : Formation of tube-like structures by vascular endothelial cells in vitro and angiogenesis in vivo. / Fukushi, Jun-Ichi; Morisaki, Takashi; Shono, Tadahisa; Nishie, Akihiro; Torisu, Hitoe; Ono, Mayumi; Kuwano, Michihiko.

In: Biochemical and Biophysical Research Communications, Vol. 250, No. 2, 18.09.1998, p. 444-448.

Research output: Contribution to journalArticle

Fukushi, Jun-Ichi ; Morisaki, Takashi ; Shono, Tadahisa ; Nishie, Akihiro ; Torisu, Hitoe ; Ono, Mayumi ; Kuwano, Michihiko. / Novel biological functions of interleukin-4 : Formation of tube-like structures by vascular endothelial cells in vitro and angiogenesis in vivo. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 250, No. 2. pp. 444-448.
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