Novel cellulose polyampholyte-gold nanoparticle-based colorimetric competition assay for the detection of cysteine and mercury(II)

Jun You, Haoze Hu, Jinping Zhou, Lina Zhang, Yaping Zhang, Tetsuo Kondo

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We provide a highly sensitive and selective assay to detect cysteine (Cys) and Hg2+ in aqueous solutions using Au nanoparticles (NPs) stabilized by carboxylethyl quaternized cellulose (CEQC). This method is based on the thiophilicity of Hg2+ and Au NPs as well as the unique optical properties of CEQC-stabilized Au NPs. CEQC chains are good stabilizing agents for Au NPs even in a high-salt solution. The addition of Cys results in the aggregation of CEQC-stabilized Au NPs, which induces the visible color change and obvious redshift in UV-visible absorption spectra. On the other hand, Hg2+ is more apt to interact with thiols than Au NPs; thus, it can remove the Cys and trigger Au NP aggregate redispersion again. By taking advantage of this mechanism, a novel off-on colorimetric sensor has been established for Cys and Hg2+ detection. This new assay could selectively detect Cys and Hg2+ with the detection limits as low as 20 and 40 nM in aqueous solutions, respectively.

Original languageEnglish
Pages (from-to)5085-5092
Number of pages8
JournalLangmuir
Volume29
Issue number16
DOIs
Publication statusPublished - Apr 23 2013

Fingerprint

cysteine
Mercury
cellulose
Cellulose
Gold
Cysteine
Assays
gold
Nanoparticles
nanoparticles
aqueous solutions
Excipients
visible spectrum
Sulfhydryl Compounds
thiols
Absorption spectra
Agglomeration
Optical properties
Salts
actuators

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Novel cellulose polyampholyte-gold nanoparticle-based colorimetric competition assay for the detection of cysteine and mercury(II). / You, Jun; Hu, Haoze; Zhou, Jinping; Zhang, Lina; Zhang, Yaping; Kondo, Tetsuo.

In: Langmuir, Vol. 29, No. 16, 23.04.2013, p. 5085-5092.

Research output: Contribution to journalArticle

You, Jun ; Hu, Haoze ; Zhou, Jinping ; Zhang, Lina ; Zhang, Yaping ; Kondo, Tetsuo. / Novel cellulose polyampholyte-gold nanoparticle-based colorimetric competition assay for the detection of cysteine and mercury(II). In: Langmuir. 2013 ; Vol. 29, No. 16. pp. 5085-5092.
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