Novel cross-linking reaction of oligonucleotides incorporating new base analogs with selective reactivity toward cytidine.

F. Nagatsugi, T. Kawasaki, M. Maeda, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

2-Amino-6-vinylpurine analog (1) has been proven to react with cytidine and guanosine in a model reaction of cross-linking. However, its incorporation into the ODNs was unsuccessful because of the high reactivity of its vinyl group. A new precursor for 1, 2-amino-6-[2-(phenylthio)ethyl]purine (2), was designed so as to generate the reactive vinyl group after introduction into the oligomers. In this paper, synthesis of ODN containing 2, easy generation of the vinyl group within the oligomer, and interstrand cross-linking between the ODN incorporating 1 and the target DNA, are described.

Original languageEnglish
Pages (from-to)67-68
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - Jan 1 1997

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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