TY - JOUR
T1 - Novel DNA/polymer conjugate for intelligent antisense reagent with improved nuclease resistance
AU - Murata, Masaharu
AU - Kaku, Wataru
AU - Anada, Takahisa
AU - Sato, Yoshikuni
AU - Kano, Takeshi
AU - Maeda, Mizuo
AU - Katayama, Yoshiki
N1 - Funding Information:
This works has been supported by grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Financial support in part was provided by the New Energy and Industrial Technology Development Organization (NEDO) to M. Murata.
PY - 2003/11/17
Y1 - 2003/11/17
N2 - Antisense technology provides an effective strategy to inhibit synthesis of the gene product. We prepared a novel antisense reagent comprised of oligodeoxynucleotides (ODN) and a thermo responsive polymer, poly(N-isopropylacrylamide) (PNIPAAm). The conjugate inhibited gene expression in a dose-dependent manner. The ODN-PNIPAAm conjugate demonstrated excellent resistance to S1 nuclease. In particular, PNIPAAm-modified antisense ODN at the 3′,5′-ends of the ODN provided complete resistance against nuclease at 37°C, which is above the phase transition temperature of the PNIPAAm side chain. These characteristics of the conjugate suggest it may have potential for use in a new gene delivery system as part of an antisense strategy.
AB - Antisense technology provides an effective strategy to inhibit synthesis of the gene product. We prepared a novel antisense reagent comprised of oligodeoxynucleotides (ODN) and a thermo responsive polymer, poly(N-isopropylacrylamide) (PNIPAAm). The conjugate inhibited gene expression in a dose-dependent manner. The ODN-PNIPAAm conjugate demonstrated excellent resistance to S1 nuclease. In particular, PNIPAAm-modified antisense ODN at the 3′,5′-ends of the ODN provided complete resistance against nuclease at 37°C, which is above the phase transition temperature of the PNIPAAm side chain. These characteristics of the conjugate suggest it may have potential for use in a new gene delivery system as part of an antisense strategy.
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U2 - 10.1016/j.bmcl.2003.08.062
DO - 10.1016/j.bmcl.2003.08.062
M3 - Article
C2 - 14592486
AN - SCOPUS:0242348711
SN - 0960-894X
VL - 13
SP - 3967
EP - 3970
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 22
ER -