Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I

N. Yamazaki, Y. Shinohara, K. Kajimoto, Mitsuru Shindo, H. Terada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

For characterization of the detailed gene structure of human muscle type carnitine palmitoyltransferase I (M-CPTI), we analyzed the 5'-upstream region of the M-CPTI transcripts. As a result, we found a cDNA clone containing a nucleotide sequence unexpected from the reported M-CPTI gene structure in the upstream region of its 5' end. Comparison of this nucleotide sequence with that of genomic DNA showed that this sequence was derived from the 3'-untranslated region of the gene encoding choline/ethanolamine kinase-β (CK/EK-β) located upstream of the M-CPTI gene. Southern blot analysis showed that there was no other region homologous to the CK/EK-β gene in the whole human genome. Thus, the overlapping transcript was concluded to be produced from the functional genes of CK/EK-β and M-CPTI. Furthermore, cDNAs containing both exons of these genes were detected by the polymerase chain reaction using the cDNA of human heart M-CPTI obtained by specific reverse transcription from its 3'-untranslated region as a template. From these results, the production and organization of these overlapping transcripts are discussed.

Original languageEnglish
Pages (from-to)31739-31746
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number41
DOIs
Publication statusPublished - Oct 13 2000
Externally publishedYes

Fingerprint

Choline Kinase
Carnitine O-Palmitoyltransferase
Choline
Muscle
Genes
Muscles
Complementary DNA
3' Untranslated Regions
Nucleotides
Gene encoding
Polymerase chain reaction
Human Genome
Transcription
Southern Blotting
ethanolamine kinase
Reverse Transcription
Exons
Myocardium
Clone Cells
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I. / Yamazaki, N.; Shinohara, Y.; Kajimoto, K.; Shindo, Mitsuru; Terada, H.

In: Journal of Biological Chemistry, Vol. 275, No. 41, 13.10.2000, p. 31739-31746.

Research output: Contribution to journalArticle

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AU - Shindo, Mitsuru

AU - Terada, H.

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