Novel fungal phenylpyruvate reductase belongs to d-isomer-specific 2-hydroxyacid dehydrogenase family

Taiki Fujii, Motoyuki Shimizu, Yuki Doi, Tomoya Fujita, Takashi Ito, Daisuke Miura, Hiroyuki Wariishi, Naoki Takaya

Research output: Contribution to journalArticle

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Abstract

We discovered the phenyllactate (PLA)-producing fungal strain Wickerhamia fluorescens TK1 and purified phenylpyruvate reductase (PPR) from fungal cell-free extracts. The PPR used both NADPH and NADH as cofactors with more preference for the former. The enzyme reaction as well as the fungal culture produced optically active d-PLA. The gene for the PPR (pprA) was cloned and expressed in Escherichia coli cells. Purified preparations of both native and recombinant PPR used hydroxyphenylpyruvate, glyoxylate and hydroxypyruvate as substrates but not pyruvate, oxaloacetate or benzoylformate. The predicted PPR protein had sequence similarity to proteins in the d-isomer-specific 2-hydroxyacid dehydrogenase family. Phylogenetic analyses indicated that the predicted PPR protein together with fungal predicted proteins constitutes a novel group of glyoxylate/hydroxypyruvate reductases. The fungus efficiently converted phenylalanine and phenylpyruvate to d-PLA. These compounds up-regulated the transcription of pprA, suggesting that it plays a role in fungal phenylalanine metabolism.

Original languageEnglish
Pages (from-to)1669-1676
Number of pages8
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1814
Issue number12
DOIs
Publication statusPublished - Dec 1 2011

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Isomers
Oxidoreductases
glyoxylate reductase
Phenylalanine
Hydroxypyruvate Reductase
Oxaloacetic Acid
Proteins
Fungal Proteins
Transcription
Cell Extracts
Fungi
Pyruvic Acid
NADP
Cell culture
Metabolism
NAD
Escherichia coli
2-hydroxyacid dehydrogenase
phenylpyruvic acid
Genes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Novel fungal phenylpyruvate reductase belongs to d-isomer-specific 2-hydroxyacid dehydrogenase family. / Fujii, Taiki; Shimizu, Motoyuki; Doi, Yuki; Fujita, Tomoya; Ito, Takashi; Miura, Daisuke; Wariishi, Hiroyuki; Takaya, Naoki.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1814, No. 12, 01.12.2011, p. 1669-1676.

Research output: Contribution to journalArticle

Fujii, Taiki ; Shimizu, Motoyuki ; Doi, Yuki ; Fujita, Tomoya ; Ito, Takashi ; Miura, Daisuke ; Wariishi, Hiroyuki ; Takaya, Naoki. / Novel fungal phenylpyruvate reductase belongs to d-isomer-specific 2-hydroxyacid dehydrogenase family. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2011 ; Vol. 1814, No. 12. pp. 1669-1676.
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