Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B

Takeshi Sekiguchi, Eiji Hirose, Nobutaka Nakashima, Miki Ii, Takeharu Nishimoto

Research output: Contribution to journalArticlepeer-review

150 Citations (Scopus)

Abstract

Rag A/Gtr1p are G proteins and are known to be involved in the RCC1-Ran pathway. We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. Rag C demonstrates homology with Rag D (81.1% identity) and with Gtr2p of Saccharomyces cerevisiae (46.1% identity), and it belongs to the Rag A subfamily of the Ras family. Rag C and Rag D contain conserved GTP-binding motifs (PM-1, -2, and -3) in their N-terminal regions. Recombinant glutathione S-transferase fusion protein of Rag C efficiently bound to both [ 3H]GTP and [3H]GDP. Rag A was associated with both Rag C and Rag D in their C-terminal regions where a potential leucine zipper motif and a coiled-coil structure were found. Rag C and D were associated with both the GDP and GTP forms of Rag A. Both Rag C and Rag D changed their subcellular localization, depending on the nucleotide-bound state of Rag A. In a similar way, the disruption of S. cerevisiae GTR1 resulted in a change in the localization of Gtr2p.

Original languageEnglish
Pages (from-to)7246-7257
Number of pages12
JournalJournal of Biological Chemistry
Volume276
Issue number10
DOIs
Publication statusPublished - Mar 9 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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