Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli

Toh Ru Shimuta, Kiyotaka Nakano, Yoko Yamaguchi, Shogo Ozaki, Kazuyuki Fujimitsu, Chika Matsunaga, Kenji Noguchi, Akiko Emoto, Tsutomu Katayama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Escherichia coli DnaA protein initiates chromosomal replication and is an important regulatory target during the replication cycle. In this study, a suppressor mutation isolated by transposon mutagenesis was found to allow growth of the temperature-sensitive dnaA508 and dnaA167 mutants at 40 °C. The suppressor consists of a transposon insertion in a previously annotated ORF, here termed hspQ, a novel heat shock gene whose promoter is recognized by the major heat shock sigma factor σ 32. Expression of hspQ on a pBR322 derivative inhibits growth of the dnaA508 and dnaA167 mutants at 30 °C, whereas growth of dnaA46 and other dnaA mutants is insensitive to changes in the level of hspQ. Cellular DnaA308 protein is degraded rapidly at elevated temperature, but hspQ disruption impedes this process. In contrast, DnaA46 protein is rapidly degraded in an hspQ-independent manner. Gel-filtration and chemical cross-linking experiments suggest that HspQ forms a stable homodimer in solution and can form homomultimers consisting of about four monomers. Heat-shock induced proteases such as Clp contain homomultimers of subunit proteins. We propose that HspQ is a new factor involved in the quality control of proteins and that it functions by excluding denatured proteins.

Original languageEnglish
Pages (from-to)1151-1166
Number of pages16
JournalGenes to Cells
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 1 2004

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli'. Together they form a unique fingerprint.

  • Cite this

    Shimuta, T. R., Nakano, K., Yamaguchi, Y., Ozaki, S., Fujimitsu, K., Matsunaga, C., Noguchi, K., Emoto, A., & Katayama, T. (2004). Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli. Genes to Cells, 9(12), 1151-1166. https://doi.org/10.1111/j.1365-2443.2004.00800.x