Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum

Tsuyoshi Yoshida, Yukihiro Tashiro, Kenji Sonomoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We previously reported a butanol production process with pH-stat continuous feeding of dl-lactic acid and glucose as the co-substrate (Oshiro et al., Appl. Microbiol. Biotechnol., 87, 1177-1185, 2010). To accomplish butanol production from completely inedible substrates, in this study, we investigated acetone-butanol-ethanol (ABE) fermentation of Clostridium saccharoperbutylacetonicum N1-4 with lactic acid by using pentose as the co-substrate. Examination for optimum co-substrate indicated that arabinose was superior to glucose and xylose for ABE fermentation. Actually batch culture with lactic acid and arabinose without pH control exhibited higher butanol production (7.11 g/l) and lactic acid consumption (2.02 g) than those (6.62 g/l and 1.45 g, respectively) with glucose. Fed-batch culture without pH control increased these values to 12.08 g/l and 15.60 g/l butanol production, and to 3.83 g and 5.91 g lactic acid consumption by feeding the substrate once and twice, respectively. Finally, the result of gas chromatography-mass spectroscopy analysis using [1,2,3-13C3]-lactic acid indicated that lactic acid was converted to butanol with the efficiency of 51.9%. Thus, we established a novel high butanol production from lactic acid using arabinose as the co-substrate in simple fed-batch culture.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume114
Issue number5
DOIs
Publication statusPublished - Nov 1 2012

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Pentoses
Clostridium
Butanols
Lactic acid
Butenes
Lactic Acid
Arabinose
Batch Cell Culture Techniques
Substrates
Glucose
Acetone
Fermentation
Ethanol
Xylose
Gas chromatography
Gas Chromatography
Mass Spectrometry
Spectroscopy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum. / Yoshida, Tsuyoshi; Tashiro, Yukihiro; Sonomoto, Kenji.

In: Journal of Bioscience and Bioengineering, Vol. 114, No. 5, 01.11.2012, p. 526-530.

Research output: Contribution to journalArticle

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